InBio is excited to offer three new human IgE monoclonal antibodies for detection of alpha-gal, the primary cause of red meat allergy and alpha-gal syndrome (AGS).

The new hIgE mAb (clones 7G6, 5E15 and 1D13) were used together with clones 10H8 and 16D9 in a recent study of alpha-gal-induced allergic effector cell activation. This study reported functional diversity in the hIgE mAb clones based on their ability to bind different alpha-gal glycoproteins (bovine thyroglobulin, cetuximab and alpha-gal-HSA). Clones 16D9 and 7G6 bound to all three glycoproteins and showed greater “breadth” of alpha gal binding than 10H8 and 1D13.

The five hIgE mAb to alpha gal showed distinct binding curves in ELISA (7G6>5E15>16D9>10H8>1D13). These antibodies recognize three unique alpha-gal glycan determinants and have IgE concentrations that are orders of magnitude higher than sera from AGS patients. InBio is developing a synthetic serum comprising the hIgE mAb as an alternative to patient sera, providing researchers with specific tools to investigate AGS.

This panel of five hIgE mAb to alpha gal is exclusively available from InBio through license agreements with Vanderbilt University Medical Center.

In Other News:

Human IgE mAb to Bet v 1

The hIgE mAb are derived from patients with a clinical history of allergic disease and serve as unambiguous reference standards for research and diagnostic purposes. A promising alternative to human sera, the current panel comprises 57 hIgE mAb to major food and inhaled allergens from dust mite, cockroach, cat, dog, pollen, peanut, milk, cashew, walnut, and egg.

The latest addition to our panel of hIgE mAb panel to inhalant allergens is an hIgE mAb to Bet v 1, the major birch pollen allergen. This mAb, 2H22, reported by O’Malley et al, binds to a distinct site from the IgG anti-Bet v 1 mAb produced by Regeneron for use as allergen-specific biologics. The hIgE mAb 2H22 also binds more strongly to rBet v 1 than to nBet v 1.

Applications for the hIgE mAb include:

• QC and validation of purified allergens for molecular diagnostics
• In vitro IgE diagnostic tests, calibrators, controls, alternatives to human sera
• Molecular reference standards for IgE
• Epitope analysis and localization of IgE epitopes
• Mast cell/basophil activation assays and histamine release
• Animal models of anaphylaxis