InBio’s New Panel of hIgE mAb!
InBio is delighted to announce that it has recently expanded its unique and exclusive product line of human IgE monoclonal antibodies (hIgE mAb). Remarkably, the panel includes two hIgE mAb to alpha-gal, the principal determinant of red meat allergy. The panel also includes 12 hIgE mAb to the immunodominant peanut allergens, Ara h 1, Ara h 2, Ara h 3 and Ara h 6.
The new panel of hIgE mAb have been extensively characterized by ELISA and immunoblotting, together with assessments of total and specific IgE by ImmunoCAP (see Table below). The panel includes hIgE mAb to distinct (non-overlapping) epitopes (as indicated by asterisks in the Table).
InBio’s hIgE mAb product line serves the allergy and immunology community as unambiguous reference standards for research and diagnostic purposes, providing a promising alternative to human sera.
The current panel comprises 28 hIgE mAb to major food and inhaled allergens from dust mite, cat, dog, peanut, cashew, walnut, egg, and alpha-gal, with more in the pipeline. The hIgE mAb are derived from allergic patients with a clinical history of allergic disease using proprietary methods. Multiple antibodies are available for specific target allergens, providing options for research applications and mechanistic studies of allergen-IgE interactions.
Applications for the hIgE mAb include:
- QC and validation of purified allergens for molecular diagnostics
- In vitro IgE diagnostic tests, calibrators, controls, alternatives to human sera
- Molecular reference standards for IgE
- Epitope analysis and localization of IgE epitopes
- Mast cell/basophil activation assays and histamine release
- Animal models of anaphylaxis
Alpha-gal Human IgE mAb
Alpha-gal Human IgE mAb
Although IgE antibodies were discovered over 50 years ago, they occur at very low concentrations in the blood of allergic patients. Previously, it was not feasible to obtain purified IgE antibodies for research on allergic diseases. A remarkable breakthrough by scientists at Vanderbilt University Medical Center harnessed the power of monoclonal antibody technology to produce unique, hIgE antibodies that can be manufactured in unlimited amounts and that are available in perpetuity.
Example data from hIgE mAb 16D9 to alpha-gal.
Research on the role of alpha gal in red meat allergy had been hindered by the lack of availability of suitable monoclonal antibodies. The two hIgE mAb to alpha gal (16D9 and 10H8) were derived from a patient who experienced severe allergic reactions after eating red meat. These antibodies show >90% purity on SDS-PAGE and show sigmoidal binding curves in ELISA using bovine thyroglobulin. The hIgE mAb to alpha gal are unique probes that can be used to investigate red meat allergy and tick borne diseases.
Research on hIgE mAb Structure
InBio continues its research in identifying critical, allergen-binding IgE epitopes due to the human IgE mAb technology. This research will help facilitate structure-based design of allergen vaccines. Learn more about this research in our latest publication on the structure and biologic activity of the 2F10 hIgE mAb epitope on Der p 2:
Khatri et al, Human IgE monoclonal antibody recognition of mite allergen Der p 2 defines structural basis of an epitope for IgE cross-linking and anaphylaxis in vivo. PNAS Nexus 2022: https://doi.org/10.1093/pnasnexus/pgac054
Let’s Work Together
Whether you conduct primary research on allergens, develop diagnostic systems, or create products to alleviate environmental and food allergies, you need expertise in action. And that’s exactly what InBio delivers, across everything from highly purified allergens and advanced test kits, to environmental and food allergen testing, to contract research for specialized needs.
- Broad product catalog
- Deep scientific background
- Ongoing commitment to R&D
- Well-documented subject-matter expertise
- Direct contact with our scientific team
- Flexible, collaborative relationships
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