InBio natural and recombinant allergens are purified by affinity chromatography and/or HPLC. Protein identity and amino acid composition are verified by mass spectrometry and by amino acid analysis. Immune reactivity is validated by monoclonal antibody binding. Purified allergens are also validated for IgE antibody binding. All of it under an ISO 9001:2015 certified Quality Management System.
Applications for InBio Purified Allergens.
- Molecular Allergy Diagnostics
- In vitro studies of T-cell function and antigen presentation
- Animal models of IgE responses & asthma
- Innate Immunity
- Antibody detection, histamine release assays
- Structural studies and biologic assays
LoTox™ Purified Allergens: Made for research.
Designed specifically for research studies where endotoxin may influence the cellular response, LoTox™ allergens have very low endotoxin levels (<0.03 EU/µg protein), making them ideal for human and murine cellular studies using T-cells, APC’s or dendritic cells.
Watch InBio’s Purified Allergens videos.
Dr.Sabina Wünschmann, VP of Allergen Manufacturing, R&D, discusses the advantages of using purified allergens, and provides insight into InBio’s allergen manufacturing processes.
