Tropomyosin ELISA kit (EL-TPM)

Tropomyosin ELISA kit (EL-TPM)
Product Code: EL-TPM
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring D. pteronyssinus tropomyosin allergen, Der p 10.
Sensitivity: 50-0.1ng/ml
Limit of Detection: 0.2ng/ml
Assay Performance: Inquire
Contents:

Monoclonal antibody 1A6, Tropomyosin Standard, Rabbit anti Shrimp Tropomyosin; sufficient reagents for 10 microtiter plates.

Storage: Store the ELISA kit at 4°C.
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Peroxidase conjugated Goat anti-Rabbit IgG
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-TPM Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

   
  Certificate of Analysis
Monoclonal Antibody: 1A6
Vial: Red Cap; 100µl
Immunogen: Mite (D. pteronyssinus) extract
Isotype: Mouse IgG1
Specificity: Binds to specific epitope present on D. pteronyssinus tropomyosin allergen, Der p 10. Cross reactive with shellfish tropomyosin.
Purification: Produced in ascites and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1.0mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Polyclonal Antibody: Rabbit anti Shrimp Tropomyosin
Vial: Brown Cap; 100µl
Immunogen: Purified natural Shrimp Tropomyosin
Specificity: The pAb contains IgG antibodies to shellfish tropomyosin.
Composition: The pAb is in phosphate buffered saline, pH 7.4, containing 1% BSA/50% glycerol.  The pAb has been 0.22µm filtered and should be diluted 1/1000 for use in the Tropomyosin ELISA.

  Certificate of Analysis
Allergen Standard: Natural Shrimp Tropomyosin
Vial: White Cap; 400µl
Composition: Purified natural Shrimp Tropomyosin prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration: 500ng/ml
Calibration:

The concentration of the purified natural shrimp tropomyosin was determined by amino acid analysis. There are no national or international reference standards for tropomyosin.

Specificity: The ELISA detects predominantly invertebrate tropomyosin from: dust mites (D. pteronyssinus and D farinae), cockroach, shrimp, crab, lobster, clam, and oyster. Mammalian or avian tropomyosin from beef, pork, lamb and turkey do not react in the assay.

References:

1.  Shanti KN, Martin BM, Nagpal S, Metcalfe DD, Subba Rao PV. Identification of tropomyosin as the major shrimp allergen and characterization of its IgE-binding epitopes. J Immunol. 1993; 151 (10):5354-5363.

2.  Daul CB, Slattery M, Reese G, Lehrer SB. Identification of the major brown shrimp (Penaeus aztecus) allergen as the muscle protein tropomyosin. Int Arch Allergy Immunol. 1994; 105(1):49-55.

3.  Jeoung BJ, Reese G, Hauck P, Oliver JB, Daul CB, Lehrer SB. Quantification of the major brown shrimp allergen Pen a 1 (tropomyosin) by a monoclonal antibody-based sandwich ELISA. J Allergy Clin Immunol. 1997; 100(2):229-234.

4.  Santos AB, Rocha GM, Oliver C, Ferriani VPL, Lima RC, Palma MS, Sales VSF, Aalberse RC, Chapman MD, Arruda LK. Crossreactive IgE antibody responses to tropomyosins from Ascaris lumbricoides and cockroach. J Allergy Clin Immunol 2008; 121 (4):1040-1046.

(For complete reference list see EL-TPM Certificate of Analysis)

 

File Name Size Added Link
EL-TPM_CoA 377.5KB 21/05/2014 Download
Certificate of Analysis for Tropomyosin ELISA kit
Tags: EL-TPM
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