Technical Information on MARIA®

What is MARIA® Technology?

The Multiplex Array for Indoor Allergens (MARIA®) combines Indoor Biotechnologies proprietary panels of monoclonal antibodies with the Luminex xMAP® multiplexing technology. MARIA® technology uses polystyrene microspheres that are internally dyed with distinct fluorophores to create as many as 100 distinctly coded bead sets. Capture antibodies are covalently coupled to different bead sets and then used to develop quantitative immunoassays using biotinylated detector antibodies and a reporting fluorophore.

Up to 11 common allergens of dust mite (Der p 1, Der f 1 and Mite Group 2), cat (Fel d 1), dog (Can f 1), mouse (Mus m 1), rat (Rat n 1), cockroach (Bla g 2), Alternaria (Alt a 1) and the pollen allergens of birch (Bet v 1) and timothy grass (Phl p 5) can currently be measured simultaneously using this technology. The detection panel is continuously being enlarged to include other allergens, such as food allergens and other molds.

Multiplex Allergen Detection Using MARIA®

MARIA® offers major advantages compared to ELISA such as the time savings achieved by analyzing multiple allergens at once, as well as improved sensitivity, accuracy and reproducibility.

Comparisons of Assay Performance between ELISA and MARIA®

Comparison of required Assay Time
Example: 200 dust extracts to be analyzed for 8 allergens

Samples per plate 18 20
Number of plates required 12 x 8 =96 10
Total technician time 16 days 3 days

Sensitivity: Lower Limit of Detection (LLOD): MARIA® is up to 40-fold more sensitive than ELISA

Allergen ELISA
Der p 1 2 0.06
Der f 1 2 0.06
Mite Group 2 0.8 0.02
Fel d 1 0.8 0.02
Can f 1 2 0.06
Mus m 1 0.2 0.01
Rat n 1 0.8 0.02
Bla g 2 2 0.98
Alt a 1 0.8 0.02
Bet v 1 2 0.2
Phl p 5 4 0.2

What can we learn from multiplex allergen testing?
Presented at the Indoor Air Quality Association annual meeting, Tampa, Florida, February 2010.

International Multi-Center Study of MARIA®
Presented at the European Academy of Allergy and Clinical Immunology (EAACI) annual meeting, Warsaw, Poland, June 2009

Quality Control procedures using MARIA
Presented at the EAACI annual meeting, London, UK, June 2010.

Earle et al. High throughput fluorescent multiplex array for indoor allergen exposure assessment. J Allergy Clin Immunol, 2007; 119:428-33 (pdf).

For further information, contact: Dr. Eva King.
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