Cry j 1 ELISA 2.0 kit - Five plate pack (EPC-CJ1-5)

Cry j 1 ELISA 2.0 kit - Five plate pack (EPC-CJ1-5)
Product Code: EPC-CJ1-5
Availability: In Stock
Price: $1,495.00
Qty:  
ELISA 2.0 kit for measuring Japanese Cedar pollen allergen, Cry j 1.
Antibodies: 4D12/2E11
Standard Range: 100-0.02 ng/ml
Limit of Detection: 0.02ng/ml
Background OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents: Plate: Pre-coated with anti-Cry j 1 monoclonal antibody 4D12
Vial 1: (white top) Cry j 1 allergen standard
Concentration: 1000 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 2E11
Vial 3: (red top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: 6 months from date of receipt
Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources: EPC-CJ1-1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plates coated with
monoclonal antibody 4D12 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 4D12
Immunogen: Cry j 1
Isotype: Mouse IgG1/Kappa
Specificity: Binds to species specific epitope present on Cryptomeria japonica allergen, Cry j 1.
Purification: Produced in vitro and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: 2E11
Immunogen: Japanese Cedar pollen extract
Isotype: Mouse IgG1/Kappa
Specificity: Binds to species specific epitope present on Cryptomeria japonica allergen, Cry j 1.
Purification: Produced in vitro and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Recombinant Cry j 1 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration: 1000 ng/mL

References:

1. Fujimura T, Kawamoto S. Spectrum of allergens for Japanese cedar pollinosis and impact of
component-resolved diagnosis on allergen-specific immunotherapy. Allergol Int 2015; 64:312
2. Hashimoto M, et al. Sensitivity to two major allergens (Cry j I and Cry j II) in patients with
Japanese cedar (Cryptomeria japonica) pollinosis. Clin Exp Allergy 1995; 9:848
3. Yasueda H, et al. A basic policy for allergen standardization in our country and
standardization of Japanese cedar (Cryptomeria japonica) pollen extracts. Allergol Int 1997;
46:135

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