Phl p 5 ELISA 2.0 kit - Single plate pack (EPC-PP5-1)

Phl p 5 ELISA 2.0 kit - Single plate pack (EPC-PP5-1)
Product Code: EPC-PP5-1
Availability: In Stock
Price: $395.00
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ELISA 2.0 kit for measuring Timothy grass pollen allergen, Phl p 5.
Antibodies: 1D11/Bo1
Standard Range: 250-0.49 ng/ml
Limit of Detection: 0.49ng/ml
Background OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents: Plate: Pre-coated with anti-Phl p 5 monoclonal antibody 1D11
Vial 1: (white top) Phl p 5a allergen standard
Concentration: 2500 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody Bo1
Vial 3: (red top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: 6 months from date of receipt
Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-PP5-5 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 1D11 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 1D11 (clone 1D11 C8)
Immunogen: Phl p 5
Isotype: Mouse IgG1
Specificity: Binds to a species specific epitope on Timothy grass pollen allergen, Phl p 5a & b.
Purification: Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: Bo1
Immunogen: Crude Timothy pollen extract
Isotype: Mouse IgG1
Specificity: Binds to species specific epitope present on Timothy Grass Pollen Allergen, Phl p 5a & b.
Purification: From tissue culture by ammonium sulphate precipitation and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Recombinant Phl p 5a prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration: 2500 ng/mL (The rPhl p 5a was from E. coli and purified by conventional biochemical methods. Validity of the rPhl p 5 standard was confirmed by comparison with the European Pharmacopoeia reference CRS (Y0001566), recombinant major allergen rPhl p 5 containing 8.56 μg of rPhl p 5 per vial.)

References:

  1. Müller WD, Diener C, Jung K, Jäger L. Antigens of Timothy and other grass pollen extracts identified by monoclonal antibodies. Allergol Immunopathol (Madr). 1988;16:315-20.
  2. Fahlbusch B, Müller WD, Diener CH, Jäger L. Detection of crossreactive determinants in grass pollen extracts using monoclonal antibodies against group IV and group V allergens. Clin Exp Allergy. 1993;23:51-60.
  3. Petersen A, Becker WM, Schlaak M. Epitope analysis of isoforms of the major allergen Phl p V by fingerprinting and microsequencing. Clin Exp Allergy. 1994;24:250-6.
  4. S. Vieths, D. Barber, M. Chapman, A. Costanzo, A. Daas, H. Fiebig, K.M. Hanschmann, M. Hrabina, S. Kaul, A. Ledesma, P. Moingeon, G. Reese, C. Schörner, R. van Ree, B. Weber, K.H. Buchheit. Establishment of recombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standards and validation of ELISA methods for their measurement. Pharmeuropa Bio&SN October 2012; 118-13.
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