Phl p 5 ELISA kit (EL-PP5)

Phl p 5 ELISA kit (EL-PP5)
Product Code: EL-PP5
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring Phleum pratense allergen, Phl p 5.
Antibodies: 1D11/Bo1
Sensitivity: 1ng/ml
Limit of Detection: 4ng/ml
Assay Performance: Inquire
Contents:

Monoclonal antibody 1D11, Phl p 5 Standard, Biotinylated antibody Bo1; sufficient reagents for 10 plates.

Storage: Store at 4°C.
Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6
Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine Serum Albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-PP5 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

   
  Certificate of Analysis
Monoclonal Antibody: 1D11 (clone 1D11 C8)
Vial: Red Cap; 100µl
Immunogen: Timothy grass pollen extract
Isotype: Mouse IgG1
Specificity:

Binds to species specific epitope present on timothy grass pollen allergen, Phl p 5a & b.

Purification: Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. 
Concentration: 1.5mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. 

  Certificate of Analysis
Monoclonal Antibody: Bo1
Vial: Brown Cap; 100µl
Immunogen: Phl p 5
Isotype: Mouse IgG1
Specificity:

Binds to species specific epitope present on timothy grass pollen allergen, Phl p 5a & b.

Purification: Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, 0.22µm filtered, preservative free. 

  Certificate of Analysis
Allergen Standard: rPhl p 5a
Vial: White Cap; 400µl
Composition: Recombinant Phl p 5 prepared in 1% BSA/50 glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.
Concentration: 5000ng/ml
Calibration: The rPhl p 5a was from E. coli and purified by conventional biochemical methods. Validity of the rPhl p 5 standard was confirmed by comparison with the European Pharmacopoeia reference CRS (Y0001566), recombinant major allergen rPhl p 5 containing 8.56 μg of rPhl p 5a per vial. 

References:

  1. Müller WD, Diener C, Jung K, Jäger L. Antigens of Timothy and othergrass pollen extracts identified by monoclonal antibodies. AllergolImmunopathol (Madr). 1988;16:315-20. ​
  2. Fahlbusch B, Müller WD, Diener CH, Jäger L. Detection of crossreactivedeterminants in grass pollen extracts using monoclonal antibodiesagainst group IV and group V allergens. Clin Exp Allergy. 1993;23:51-60. 
  3. Petersen A, Becker WM, Schlaak M. Epitope analysis of isoforms of themajor allergen Phl p V by fingerprinting and microsequencing. Clin ExpAllergy. 1994;24:250-6. 
  4. S. Vieths, D. Barber, M. Chapman, A. Costanzo, A. Daas, H. Fiebig, K.M.Hanschmann, M. Hrabina, S. Kaul, A. Ledesma, P. Moingeon, G. Reese,C. Schörner, R. van Ree, B. Weber, K.H. Buchheit. Establishment ofrecombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. Referencestandards and validation of ELISA methods for their measurement.Pharmeuropa Bio&SN October 2012; 118-13. 
(For complete reference list see EL-PP5 Certificate of Analysis)

 

File Name Size Added Link
EL-PP5_CoA 379.36KB 28/05/2014 Download
Certificate of Analysis for Phl p 5 ELISA kit
    54.167.218.210
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