Bet v 1 ELISA 2.0EP kit - Twenty-five plate pack (EPC-BV1-EP25)

Bet v 1 ELISA 2.0EP kit - Twenty-five plate pack (EPC-BV1-EP25)
Product Code: EPC-BV1-EP25
Availability: In Stock
Price: $9,395.00
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ELISA 2.0EP kit for measuring Birch pollen allergen, Bet v 1.

Antibodies: 5B4/6H4
Standard Range: 100-0.02 ng/ml
Limit of Detection: 0.02ng/ml
Background OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents: Plate: Pre-coated with anti-Bet v 1 monoclonal antibody 5B4
Vial 1: (white top) Bet v 1 allergen standard
Concentration: 1000 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 6H4
Vial 3: (red top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: 6 months from date of receipt
Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-BV1-EP25 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 4B10 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 5B4 (clone 5B4)
Immunogen: Birch pollen extract
Isotype: Mouse IgG1
Specificity: Recognizes distinct epitope on Betula verrucosa allergen, Bet v 1. Minimal cross-reactivity with PR-10 family of pollen allergens.
Purification: Produced in tissue culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: 6H4 (clone 6H4)
Immunogen: Birch pollen extract
Isotype: Mouse IgG1
Specificity: Recognizes distinct epitope on Betula verrucosa allergen, Bet v 1. Minimal cross-reactivity with PR-10 family of pollen allergens.
Purification: Produced in tissue culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Recombinant Bet v 1a prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration: 1000 ng/mL (The rBet v 1a was produced from Pichia and purified by a multi-step chromatography procedure. Validity of the rBet v 1 standard was confirmed by comparison with the European Pharmacopoeia reference CRS (Y0001565), recombinant major allergen rBet v 1 containing 10.25μg of rBet v 1 per vial.)

References:

  1. Kaul S, et al. Standardization of allergen products: 3. Validation of candidate European Pharmacopoeia standard methods for quantification of major birch allergen Bet v 1. Allergy. 2016; 71:1414.

  2. Vieths S, et al. Establishment of recombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standards and validation of ELISA methods for their measurement. Results from feasibility studies. Pharmeur Bio Sci Notes. 2012; 2012:118.

  3. Himly M, et al. Standardization of allergen products: 1. Detailed characterization of GMP-produced recombinant Bet v 1.0101 as biological reference preparation. Allergy. 2009; 64:1038.

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