Bet v 1 ELISA kit (EL-BV1)

Bet v 1 ELISA kit (EL-BV1)
Product Code: EL-BV1
Availability: In Stock
Price: $795.00
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ELISA kit for measuring Betula verrucosa allergen, Bet v 1.
Antibodies: 4B10/2E10
Sensitivity: 0.5ng/ml
Limit of Detection: 2ng/ml
Assay Performance: Inquire
Contents:

Monoclonal antibody 4B10, Bet v 1 Standard, Biotinylated antibody 2E10; sufficient reagents for 10 plates.

Storage: Store at 4°C.
Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6
Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine Serum Albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-BV1 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Monoclonal Antibody: 4B10 (clone 4B10)
Vial: Red Cap; 100µl
Immunogen: Bet v 1
Isotype: Mouse IgG1
Specificity: Binds to multiple isoforms of Betula verrucosa allergen, Bet v 1.
Purification: Protein A affinity chromatography
Concentration: 1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. 

  Certificate of Analysis
Monoclonal Antibody: 2E10 (clone 2E10 G6 G7)
Vial: Brown Cap; 100µl
Immunogen: Bet v 1
Isotype: Mouse IgG1
Specificity: Binds to a specific epitope on Betula verrucosa allergen, Bet v 1.
Purification: Enrichment and concentration using 50% saturated ammonium sulphate precipitation followed by purification using protein A affinity chromatography.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. 

  Certificate of Analysis
Allergen Standard: rBet v 1a
Vial: White Cap; 400µl
Composition: Recombinant Bet v 1a prepared in 1% BSA/50 glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.
Concentration: 2500ng/ml Bet v 1
Calibration: The rBet v 1a was produced from Pichia and purified by a multi-step chromatography procedure. Validity of the rBet v 1 standard was confirmed by comparison with the European Pharmacopoeia reference CRS (Y0001565), recombinant major allergen rBet v 1 containing 10.25μg of rBet v 1 per vial. 
References:  

1. Breiteneder H, Pettenburger K, Bito A, et al. The gene coding for themajor birch pollen allergen Bet v 1, is highly homologous to a peadisease resistance response gene. EMBO J. 1989;8:1935-8. 

2. Hoffmann-Sommergruber K, Susani M, Ferreira F, et al. High-levelexpression and purification of the major birch pollen allergen, Bet v 1.Protein Expr Purif. 1997;9:33-9. 

3. Godnic-Cvar J, Susani M, Breiteneder H, et al. Recombinant Bet v 1, themajor birch pollen allergen, induces hypersensitivity reactions equal tothose induced by natural Bet v 1 in the airways of patients allergic to treepollen. J Allergy Clin Immu nol. 1997;99:354-9. 

4. Schappi GF, Monn C, Wuthrich B, Wanner HU. Direct determination ofallergens in ambient aerosols: methodological aspects. Int Arch AllergyImmunol. 1996;110:364-70. 

5. Schappi GF, Suphioglu C, Taylor PE, Knox RB. Concentrations of themajor birch tree allergen Bet v 1 in pollen and respirable fine particles inthe atmosphere. J Allergy Clin Immunol. 1997;100:656-61. 

6. S. Vieths, D. Barber, M. Chapman, et al. Establishment of recombinantmajor allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standardsand validation of ELISA methods for their measurement. Pharmeuropa Bio&SN October 2012;118-13. 

(For complete reference list see EL-BV1 Certificate of Analysis)

 

File Name Size Added Link
EL-BV1_CoA 344.51KB 12/05/2014 Download
Certificate of Analysis for Bet v 1 ELISA kit
    54.80.21.61
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