The IgE-QBA™ unites INDOOR Biotechnologies MARIA® testing platform and its repertoire of purified natural and recombinant allergens to create a flexible, fully-customizable quantitative assay for detection of multiple IgE analytes in human samples. At this time, the array is available as beta-version, for research use only.
IgE-QBA™ Array features:
Simultaneous detection of multiple IgE analytes in a single test well (Luminex xMAP® platform)
Quantitative results reported in IU/mL
High-throughput system: analyze 22 human samples for up to 11 analytes on one 96-well plate
Maximum flexibility: Select any combination of these 12 avaliable analytes to create your customized panel (1- to 11-plex):
Each kit may be used for analysis of either Der p 2 or Der f 2.
Panels for peanut- and cockroach-specific IgE are in development.
Click here to view IgE-QBA™ performance characteristics and result comparisons with Streptavidin-ImmunoCAP. (Presented at the AAAAI 2013.) To view the product insert with assay protocol, technical notes and data handling instructions, click here.
The Data Analysis Reference sheet for the IgE-QBA™ protocol is available here
How does the IgE-QBA™ work?
Antibody-coupled microspheres (1) are coated with purified allergen (2)
Human plasma or serum is added (3)
IgE is detected using a biotinylated antibody (4) and a streptavidin-coupled fluorophore (5)
Raw data is interpolated using a standard curve to yield quantitative results.