Natural Der p 1 Molecular Reference Standard (MRS-NDP1)

Natural Der p 1 Molecular Reference Standard (MRS-NDP1)
Product Code: MRS-NDP1
Availability: In Stock
Price: $595.00
Qty:  
  The Natural Der p 1 MRS is intended to serve as reference standard to determine the Der p 1 content of allergen preparations from house dust mite (Dermatophagoides pteronyssinus) by immunoassay. 
Allergen: Natural Der p 1 (Dermatophagoides pteronyssinus allergen 1)
Source: D. pteronyssinus culture  
Mol. Wt: 24 kD
Purification:

From spent mite culture by multi-step affinity chromatography. 

Composition: 10µg natural Der p 1, determined by Amino Acid Analysis, freeze dried in sealed glass vial.
SDS-PAGE: See inset. Silver-stained SDS-PAGE under non-reducing conditions shows a single band at 24 kD.
ELISA: Immunoreactive in Der p 1 specific ELISA. No trace contamination with Der p 2 was detected by ELISA.
Purity: >95% purity by in-solution LC-MS/MS after tryptic digest.
Isoforms: Analyzed by LC-MS/MS. Main Isoforms: Der p 2.0110 (43%) and Der p 2.0101 (37%). Other Isoforms present (20%): Der p 2.0103, Der p 2.0104, Der p 2.0114.
Formulation:

Prior to lyophilization, natural Der p 1 was adjusted to 50 mM volatile ammonium bicarbonate with 3% trehalose. 

 

Storage: Store at -20ºC
Product Resources: Natural Der p 1 Molecular Reference Standard Certificate of Analysis
The Natural Der p 1 MRS is an InbioTM product.
Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.
Reconstitution:
  • Allow vial to reach room temperature before use
  • Tap vial gently to collect all material at the bottom
  • Using a sterile syringe reconstitute the MRS to desired concentration by injecting a suitable volume of a buffer of choice (e.g. PBS, pH 7.4 or 1% BSA/50% glycerol/PBS, pH 7.4). 
  • Mix by gently swirling the vial until content is completely dissolved. 
  • Adding 1ml of buffer will result in a Der p 1 concentration of 10,000ng/ml. 
References:  

 

  1. Gough L, Schultz O, Sewell HF, Shakib F. The cysteine protease activity of the major house dust mite allergen Der p 1 selectively enhances the immunoglobulin E antibody responses.  J Exp Med 1999; 190:1897-1901.
  2. Wan H, Winton HL, Soeller C, Tovey ER, Gruenert DC, Thompson PJ, Stewart GA, Taylor GW, Garrod DR, Cannell MD, Robinson C.  Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions.  J Clin Invest 1999; 104:123-133.
  3. Chruszcz M, Chapman MD, Vailes LD, Stura EA, Saint-Remy JM, Minor W, Pomés A. Crystal structures of mite allergens Der f 1 and Der p 1 reveal differences in surface- exposed residues that may influence antibody binding. J Mol Biol. 2009; 386:520-530.
  4. Chapman MD et al. The European Union CREATE project: a model for international standardization of allergy diagnostics and vaccines. J Allergy Clin Immunol. 2008;122:882-889.
  5. van Ree R et al. The CREATE project: development of certified reference materials for allergenic products and validation of methods for their quantification. Allergy. 2008;63(3):310-26.
  6. Kaul S et al. Regulatory environment for allergen-specific immunotherapy. Allergy 2011;66:753-64.
  7. Chapman MD and Briza P. Molecular approaches to allergen standardization. Curr Allergy Asthma Rep. 2012;12:478-84.
File Name Size Added Link
MRS-NDP1_CoA 200.62KB 24/01/2017 Download
Natural Der p 1 Molecular Reference Standard (MRS-NDP1) Certificate of Analysis
    107.22.61.99
© 2017 INDOOR Biotechnologies, Inc.      Find us on: