Natural Der f 1 Molecular Reference Standard (MRS-NDF1)

Natural Der f 1 Molecular Reference Standard (MRS-NDF1)
Product Code: MRS-NDF1
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Price: $595.00
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  The Natural Der f 1 MRS is intended to serve as reference standard to determine the Der f 1 content of allergen preparations from house dust mite (Dermatophagoides farinae) by immunoassay
Allergen: NNatural Der f 1 (Dermatophagoides farinae allergen 1)
Source: D. farinae culture 
Mol. Wt: 24 kD
Purification:

From spent mite culture by multi-step affinity chromatography. 

Composition: 10µg natural Der f 1, determined by Amino Acid Analysis, freeze dried in sealed glass vial.
SDS-PAGE: See inset. Silver-stained SDS-PAGE under non-reducing conditions shows a single band at 24 kD.
ELISA: Immunoreactive in Der f 1 specific ELISA. No trace contamination with Der f 2 was detected by ELISA.
Purity: >95% purity by in-solution LC-MS/MS after tryptic digest.
Formulation:

Prior to lyophilization, natural Der f 1 was adjusted to 50 mM volatile ammonium bicarbonate with 3% trehalose. 

Storage: Store at -20ºC
Product Resources: Natural Der f 1 Molecular Reference Standard Certificate of Analysis
The Natural Der f 1 MRS is an InbioTM product.
Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.
Reconstitution:
  • Allow vial to reach room temperature before use
  • Tap vial gently to collect all material at the bottom
  • Using a sterile syringe reconstitute the MRS to desired concentration by injecting a suitable volume of a buffer of choice (e.g. PBS, pH 7.4 or 1% BSA/50% glycerol/PBS, pH 7.4). 
  • Mix by gently swirling the vial until content is completely dissolved. 
  • Adding 1ml of buffer will result in a Der f 1 concentration of 10,000ng/ml. 
References:  

 

  1. Lombardero M et al.  Conformational stability of B cell epitopes on Group I and Group II Dermatophagoides spp. allergens.  Effect of thermal and chemical denaturation on the binding of murine IgG and human IgE antibodies.  J Immunol 1990; 144:1353-1360.
  2. Schultz O et al.  A sensitive fluorescent assay for measuring the cysteine protease activity of Der p 1, a major allergen from the dust mite Dermataphagoides pteronyssinus.  J Clin Pathol: Mol Pathol 1998;51:222-231.
  3. Chapman MD et al. The European Union CREATE project: a model for international standardization of allergy diagnostics and vaccines. J Allergy Clin Immunol. 2008;122:882-889.
  4. van Ree R et al. The CREATE project: development of certified reference materials for allergenic products and validation of methods for their quantification. Allergy. 2008;63(3):310-26.
  5. Kaul S et al. Regulatory environment for allergen-specific immunotherapy. Allergy 2011;66:753-64.
  6. Chapman MD and Briza P. Molecular approaches to allergen standardization. Curr Allergy Asthma Rep. 2012;12:478-84.
  7. Chapman MD et al. Technological Innovations for High-Throughput Approaches to In Vitro Allergy Diagnosis. Curr Allergy Asthma Rep. 2015;15:36.
File Name Size Added Link
MRS-NDF1_CoA 197.02KB 24/01/2017 Download
Natural Der f 1 Molecular Reference Standard Certificate of Analysis
    107.22.61.99
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