Alt a 1 ELISA kit (EL-AA1)

Alt a 1 ELISA kit (EL-AA1)
Product Code: EL-AA1
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring Alternaria alternata allergen, Alt a 1.
Antibodies: 2C10/3B6
Sensitivity: 0.2ng/ml
Limit of Detection: 0.4ng/ml
Assay Performance: Inquire
Contents:

Monoclonal antibody 2C10, rAlt a 1 Standard, Biotinylated antibody 3B6; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C.
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources:

EL-AA1 Certificate of Analysis

Focus on... Alt a 1

Letter on new antibodies in Alt a 1 ELISA kit

For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Monoclonal Antibody: 2C10 (clone 2C10 G4)
Vial: Red Cap; 100µl
Immunogen: Alt a 1
Isotype: Mouse IgG
Specificity: Binds to a species specific epitope on Alternaria alternata allergen, Alt a 1.
Purification: Produced in vitro cell culture and purified by chromatography using recombinant protein G. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Monoclonal Antibody: 3B6 (clone 3B6 H9)
Vial: Brown Cap; 100µl
Immunogen: rAlt a 1
Isotype: Mouse IgG
Specificity: Binds to a species specific epitope on Alternaria alternata allergen, Alt a 1.
Purification: Produced in vitro cell culture and purified by chromatography using recombinant protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: rAlt a 1
Vial: White Cap; 400µl
Composition: Recombinant Alt a 1 prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.
Concentration: 1000ng/ml Alt a 1
Calibration: The rAlt a 1 was purified from Pichia pastoris by size exclusion HPLC. The protein content was determined by amino acid analysis.
References:  
1. Vailes L, Sridhara S, Cromwell O, Weber B, Breitenbach M, Chapman M. Quantification of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products. J Allergy Clin Immunol 2001;107(4):641-6. 
 
2. Unger A, Stoger P, Simon-Nobbe B, Susani M, Crameri R, Ebner C et al. Clinical testing of recombinant allergens of the mold Alternaria alternata. Int Arch Allergy Immunol 1999;118(2-4):220-1. 
 
3. Asturias JA, Ibarrola I, Ferrer A, Andreu C, Lopez-Pascual E, Quiralte J et al. Diagnosis of Alternaria alternata sensitization with natural and recombinant Alt a 1 allergens. J Allergy Clin Immunol 2005;115(6):1210-7. 
 
4. Chruszcz M, Chapman MD, Osinski T, Solberg R, Demas M, Porebski PJ et al. Alternaria alternata allergen Alt a 1: A unique beta-barrel protein dimer found exclusively in fungi. J Allergy Clin Immunol 2012;130(1):241-7.
(For complete reference list see EL-AA1 Certificate of Analysis)

 

File Name Size Added Link
Focus on... Alt a 1 253.48KB 22/05/2014 Download
Focus on... Alt a 1
EL-AA1-CoA 376.45KB 28/05/2014 Download
Certificate of Analysis for Alt a 1 ELISA kit
    54.167.218.210
© 2017 INDOOR Biotechnologies, Inc.      Find us on: