Ara h 1 ELISA 2.0 kit - Single plate (EPC-AH1-1)

Ara h 1 ELISA 2.0 kit - Single plate (EPC-AH1-1)
Product Code: EPC-AH1-1
Availability: In Stock
Price: $395.00

ELISA 2.0 kit for measuring Arachis hypogaea allergen, Ara h 1.

Antibodies: 2C12/2F7
Standard Range: 2,000-4.0 ng/mL
Limit of Detection: 31.5 ng/mL
Coefficient of Determination:  

Plate: Pre-coated with anti-Ara h 1 monoclonal antibody 2C12
Vial 1: (white top) Ara h 1 allergen standard
Concentration: 20,000 ng/ml
Vial 2: (brown) anti-Ara h 1 antibody 2F7
Vial 3: (red top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: 6 months from date of receipt
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-AH1-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 2C12 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 2C12
Immunogen: Ara h 1
Isotype: Mouse IgG1
Specificity: Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1.
Purification: Produced in vitro by BioVectra dcl bioreactors and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: 2F7
Immunogen: Ara h 1
Isotype Mouse IgG1
Specificity: Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1.
Activity: Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Purified natural Ara h 1 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration/Calibration: 20,000 ng/mL (based on amino acid analysis)
1.  Pomés A, Helm RM, Bannon GA, Burks AW, Tsay A, Chapman MD. Monitoring peanut allergen in food products by measuring Ara h 1. J Allergy Clin Immunol 2003;111:640-5.

2.  Pomés A, Vinton R, Chapman MD. Peanut allergen (Ara h 1) detection in foods containing chocolate. J Food Prot. 2004 Apr:67 (4):793-8.

3.  Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6.

4.  Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.



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