Mite Group 2 ELISA kit (EL-D2)

Mite Group 2 ELISA kit (EL-D2)
Product Code: EL-D2
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring Dermatophagoides spp. Group 2 allergens, Der p 2 and Der f 2.
Antibodies: 1D8/7A1
Sensitivity: 0.2ng/ml
Limit of Detection: 0.8ng/ml
Assay Performance: Intra-assay CV% of 8.9; Inter-assay CV% of 30
Contents:

Monoclonal antibody 1D8, Universal Allergen Standard, Biotinylated antibody 7A1; sufficient reagents for 10 plates.

Storage: Store at 4°C.
Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6
Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine Serum Albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-D2 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
   
  Certificate of Analysis
Monoclonal Antibody: 1D8 (clone 1D8 C4 D8)
Vial: Red Cap; 100µl
Immunogen: Der p 2
Isotype: Mouse IgG2A
Specificity: Binds to a common epitope on mite Dermatophagoides spp. Group 2 allergens, Der p 2 and Der f 2.
Purification: From ascites by ammonium sulphate precipitation and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1.35mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22µm filtered, preservative free.

  Certificate of Analysis
Monoclonal Antibody: 7A1 (clone 7A1 H1 G3)
Vial: Brown Cap; 100µl
Immunogen: Der f 2
Isotype: Mouse IgG1
Specificity: Binds to a common epitope on mite Dermatophagoides spp Group 2 allergens (Der f 2 and Der p 2).
Purification: Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Universal Allergen Standard
Vial: White Cap; 400µl
Composition: A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration:  
Universal Allergen Standard
Protein Measurement
Concentration (ng/ml)
Der p 1
Amino-acid analysis
2500
Der f 1
Amino-acid analysis
2500
Der p 2
Amino-acid analysis
1000
Fel d 1
Amino-acid analysis
1000
Can f 1
Amino-acid analysis
2500
Rat n 1
Amino-acid analysis
1000
Mus m 1
Amino-acid analysis
250
Bla g 2
Amino-acid analysis
2500

References:

1.  Heymann PW, Chapman MD, Aalberse RC, Fox JW, Platts-Mills TAE.  Antigenic and structural analysis of group II allergens (Der p II and Der f II) from house dust mites (Dermatophagoides spp.).  J Allergy Clin Immunol 1989;83:1055-1067.

2.  Lombardero M, Heymann PW, Platts-Mills TAE, Fox JW, Chapman MD.  Conformational stability of B cell epitopes on Group I and Group II Dermatophagoides spp. allergens.  J Immunol 1990;144:1353-1360.

3.  DeBlay F, Heymann PW, Chapman MD, Platts-Mills TAE.  Airborne dust mite allergens:  comparison of Group II allergens with Group I mite allergen and cat allergen, Fel d I.  J Allergy Clin Immunol 1991;88:919-926.

4.  Ovsyannikova IG, Vailes LD, Li Y, Heymann PW, Chapman MD.  Monoclonal antibodies to Group II Dermatophagoides spp. allergens:  Murine immune response, epitope analysis and development of a two-site ELISA.  J Allergy Clin Immunol 1994;94:537-546.

5.  Platts-Mills TAE, Vervloet D, Thomas WR, Aalberse RC, Chapman MD.  Indoor Allergens and Asthma: Report of the Third International Workshop.  J Allergy Clin Immunol, 1997; 100:S1

(For complete reference list see EL-D2 Certificate of Analysis)

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EL-D2_CoA 386.93KB 22/05/2014 Download
Certificate of Analysis for Mite Group 2 ELISA kit
    54.81.178.153
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