Der f 2 ELISA kit (EL-DF2)

Der f 2 ELISA kit (EL-DF2)
Product Code: EL-DF2
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring D. farinae allergen, Der f 2.
Antibodies: 1D8/7A1
Sensitivity:

0.5ng/ml

Limit of Detection: 2ng/ml
Contents:

Monoclonal antibody 1D8, Der f 2 Standard, Biotinylated antibody 7A1; sufficient reagents for 10 plates.

Storage: Store at 4°C.
Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6
Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine Serum Albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-DF2 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Monoclonal Antibody:

1D8 (clone 1D8 C4 D8)

Vial: Red Cap; 100µl
Immunogen: Der p 2
Isotype: Mouse IgG2A
Specificity:

Binds to a common epitope on mite Dermatophagoides spp. Group 2 allergens, Der p 2 and Der f 2. 

Purification:

From ascites by ammonium sulphate precipitation and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

Concentration:

1.35mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG  (1.42=1mg/ml), 0.22μm filtered, preservative free.


  Certificate of Analysis
Monoclonal Antibody:

Biotin 7A1 (clone 7A1 H1 G3)

Vial: Brown Cap; 100µl
Immunogen: Der f 2
Isotype: Mouse IgG1
Specificity:

Binds to a common epitope on mite Dermatophagoides spp Group 2 allergens (Der f 2 and Der p 2). 

Purification:

Produced in ascites and purified by affinity chromatography Protein A. Single heavy and light chain bands on SDS-PAGE.  

Biotinylation:

Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. 


  Certificate of Analysis
Allergen Standard:

Natural Der f 2

Vial: White Cap; 400µl
Composition:

Prepared in 1% BSA/50% glycerol/PBS, 0.22µm filtered, preservative free, pH 7.4.

Concentration:

2500ng/ml Der f 2

Calibration:

Natural Der f 2 >95% pure by SDS-PAGE analysis. Protein concentration determined by amino acid analysis.

References:

1. Heymann PW, Chapman MD, Aalberse RC, Fox JW, Platts-Mills TAE. Antigenic and structural analysis of group II allergens (Der p II and Der f II) from house dust mites (Dermatophagoides spp.). J Allergy Clin Immunol 1989;83:1055-1067.

2. Lombardero M, Heymann PW, Platts-Mills TAE, Fox JW, Chapman MD. Conformational stability of B cell epitopes on Group I and Group II Dermatophagoides spp. allergens. J Immunol 1990;144:1353-1360.

3. DeBlay F, Heymann PW, Chapman MD, Platts-Mills TAE. Airborne dust mite allergens:  comparison of Group II allergens with Group I mite allergen and cat allergen, Fel d I. J Allergy Clin Immunol 1991;88:919-926.

4. Ovsyannikova IG, Vailes LD, Li Y, Heymann PW, Chapman MD. Monoclonal antibodies to Group II Dermatophagoides spp. allergens:  Murine immune response, epitope analysis and development of a two-site ELISA. J Allergy Clin Immunol 1994;94:537-546.

5. Platts-Mills TAE, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor Allergens and Asthma: Report of the Third International Workshop. J Allergy Clin Immunol, 1997; 100:S1

6. van Ree R, Chapman MD, Ferreira F, Vieths S, Bryan D, et al. The CREATE project: development of certified reference materials for allergenic products and validation of methods for their quantification. Allergy 2008; 63:310-26.

7. Chapman MD, Ferreira F, Villalba M, Cromwell O, Bryan D, Becker WM, Fernández-Rivas M, Durham S, Vieths S, van Ree R; CREATE consortium. The European Union CREATE Project: A model for international standardization of allergy diagnostics and vaccines. J Allergy Clin Immunol 2008; 122:882-889.

(For complete reference list see EL-DF2 Certificate of Analysis)

File Name Size Added Link
EL-DF2_CoA 379.13KB 22/05/2014 Download
Certificate of Analysis for Der f 2 ELISA kit
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