Der f 1 ELISA kit (EL-DF1)

Der f 1 ELISA kit (EL-DF1)
Product Code: EL-DF1
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring D. farinae allergen, Der f 1.
Antibodies: 6A8/4C1
Sensitivity: 0.5ng/ml
Limit of Detection: 2ng/ml
Assay Performance: Intra-assay CV% of 7.1; Inter-assay CV% of 15
Contents: Monoclonal antibody 6A8, Universal Allergen Standard, Biotinylated antibody 4C1; sufficient reagents for 10 plates.
Storage: Store at 4°C.
Not Provided: 50mM Carbonate/Bicarbonate buffer, pH 9.6
Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine Serum Albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2
Notes:  
Product Resources: EL-DF1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Monoclonal Antibody: 6A8 (clone 6A8 B10 D12)
Vial: Red Cap; 100µl
Immunogen: Der f 1
Isotype: Mouse IgG1
Specificity: Binds to a species specific epitope present on Dermatophagoides farinae allergen, Der f 1.
Purification: Produced in tissue culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22µm filtered, preservative free.

  Certificate of Analysis
Monoclonal Antibody: 4C1 (clone 4C1 B8 3F8)
Vial: Brown Cap; 100µl
Immunogen: Der f 1
Isotype: Mouse IgG1
Specificity: Binds to a common epitope present on mite Dermatophagoides Group 1 allergens (Der f 1, Der p 1, Der m 1, Eur m 1).
Purification: Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Universal Allergen Standard
Vial: White Cap; 400µl
Composition: A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4
Concentration/Calibration:  
Universal Allergen Standard
Protein Measurement
Concentration (ng/ml)
Der p 1
Amino-acid analysis
2500
Der f 1
Amino-acid analysis
2500
Der p 2
Amino-acid analysis
1000
Fel d 1
Amino-acid analysis
1000
Can f 1
Amino-acid analysis
2500
Rat n 1
Amino-acid analysis
1000
Mus m 1
Amino-acid analysis
250
Bla g 2
Amino-acid analysis
2500

References:

1.   Chapman, M.D., Sutherland, W.M., and Platts-Mills, T.A.E. (1984).  Recognition of two Dermatophagoides pteronyssinus specific epitopes on Antigen P1 using  monoclonal antibodies:  Binding to each epitope can be inhibited be sera from mite allergic patients.  J. Immunol. 133:2488-2495.

2.  Ford, A.W., Rawle, F.C., Lind, P., Spieksma, F.Th.M., Lowenstein, H. and Platts-Mills, T.A.E. (1985). Standardization of Dermatophagoides pteronyssinus: Assessment of potency and allergen content in ten coded extracts. Int. Archs. Allergy Appl. Immunol. 76:58-68.

3.  Heymann, P.W., Chapman, M.D. and Platts-Mills, T.A.E. (1986). Antigen Der f 1 from the dust mite Dermatophagoides farinae: Structural comparison with Der p 1 from D. pteronyssinus and epitope specificity of murine IgG and human IgE antibodies. J. Immunol. 137:2841-2847.

4.   Chapman, M.D., Heyman, P.W., Wilkins, S.R., Brown, M.J., and Platts-Mills, T.A.E. (1987). Monoclonal immunoassays for major dust mite (Dermatophagoides) allergens, Der p 1 and Der f 1, and quantitative analysis of the allergen content of mite and house dust extracts. J. Allergy Clin. Immunol. 80:184-194.

5.  Platts-Mills, T.A.E. and Chapman, M.D. (1987). Dust mites: Immunology, allergic disease and environmental control. J. Allergy Clin. Immunol. 80:755-775

6.  Chapman, M.D., Heymann, P.W., and Platts-Mills, T.A.E. (1987). Epitope mapping of two major inhalant allergens, Der p 1 and Der f 1, from mites of the genus Dermatophagoides. J. Immunol. 139: 1479-1484.

7.  Chapman, M.D. (1988). Allergen specific monoclonal antibodies: New tools for the management of allerigic disease. Allergy 43 (5):7-14.

8.  Luczynska, C.M., Arruda, L.K., Platts-Mills, T.A.E., Miller, J.D., Lopez, M., and Chapman, M.D. (1989). A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p 1 and Der f 1. J. Immunol. Meths. 118:227-235.

9.  Platts-Mills T.A.E., Thomas W.R., Aalberse R.C., Vervloet D. and Chapman M.D. (1992). Dust mite allergens and asthma: Report of a second international workshop. J Allergy Clin Immunol 89:1046-1060.

10.  Platts-Mills T.A.E., Vervloet D., Thomas W.R., Aalberse R.C., Chapman M.D. Indoor Allergens and Asthma: Report of the third international workshop. J Allergy Clin Immunol, 1997; 100:S1-S24.

(For complete reference list see EL-DF1 Certificate of Analysis)

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EL-DF1_CoA 388.05KB 21/05/2014 Download
Certificate of Analysis for Der f 1 ELISA kit
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