Ara h 3 ELISA kit (EL-AH3)

Ara h 3 ELISA kit (EL-AH3)
Product Code: EL-AH3
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring Arachis hypogaea allergen, Ara h 3.
Antibodies: 1E8/4G9
Sensitivity: 0.2ng/ml
Limit of Detection: 2.0ng/ml
Assay Performance:  
Contents:

Monoclonal antibody 1E8, Ara h 3 Allergen Standard, Biotinylated antibody 4G9; sufficient reagents for 10 microtiter plates.

Storage: Store antibodies at 4°C; store ST-AH3 at -20°C upon arrival.
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-AH3 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Monoclonal Antibody: 1E8
Vial: Red Cap; 100µl
Immunogen: Ara h 3
Isotype: Mouse IgG1
Specificity: Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 3.
Purification: Produced in vitro and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1.1 mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Monoclonal Antibody: 4G9
Vial: Brown Cap; 100µl
Immunogen: Ara h 3
Isotype: Mouse IgG1
Specificity: Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 3.
Purification: Produced in vitro and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: nAra h 3
Vial: White Cap; 400µl
Composition: Naturally purified Ara h 3 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration: 1,250ng/ml Ara h 3
Calibration: The Ara h 3 concentration of the purified Ara h 3 was determined by amino acid analysis.
Stability/Storage: Please store the standard at -20ºC (+/- 5°C).

References:

1.  Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6.

2.  Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.

(For complete reference list see EL-AH3 Certificate of Analysis)

 

File Name Size Added Link
Focus on... Peanut Allergens 317.68KB 22/05/2014 Download
Focus on... Peanut Allergens
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