Ara h 1 ELISA kit (EL-AH1)

Ara h 1 ELISA kit (EL-AH1)
Product Code: EL-AH1
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring Arachis hypogaea allergen, Ara h 1.
Antibodies: 2C12/2F7
Sensitivity: 4ng/ml
Limit of Detection: 31.5ng/ml
Assay Performance: Intra-assay CV% of 3.1; Inter-assay CV% of 11.8
Contents:

Monoclonal antibody 2C12, Ara h 1 Allergen Standard, Biotinylated antibody 2F7; sufficient reagents for 10 microtiter plates.

Storage: Store antibodies at 4°C; store ST-AH1 at -20°C upon arrival (standard has 3 month shelf life).
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-AH1 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Monoclonal Antibody: 2C12 (clone 2C12 A11 A3)
Vial: Red Cap; 100µl
Immunogen: Ara h 1
Isotype: Mouse IgG1
Specificity: Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1.
Purification: Produced in vitro by BioVectra dcl bioreactors and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
Concentration: 2.7 mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Monoclonal Antibody: 2F7 (clone 2F7 C12 D10)
Vial: Brown Cap; 100µl
Immunogen: Ara h 1
Isotype: Mouse IgG1
Specificity: Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1.
Purification: Produced in ascites and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: nAra h 1
Vial: White Cap; 400µl
Composition: Naturally purified Ara h 1 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration: 20,000ng/ml Ara h1
Calibration: The Ara h 1 concentration of the purified Ara h 1 was determined by OD280.
Stability/Storage: Please store the standard at -20ºC (+/- 5°C).

References:

1.  Pomés A, Helm RM, Bannon GA, Burks AW, Tsay A, Chapman MD. Monitoring peanut allergen in food products by measuring Ara h 1. J Allergy Clin Immunol 2003;111:640-5.

2.  Pomés A, Vinton R, Chapman MD. Peanut allergen (Ara h 1) detection in foods containing chocolate. J Food Prot. 2004 Apr:67 (4):793-8.

3.  Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6.

4.  Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.

(For complete reference list see EL-AH1 Certificate of Analysis)

 

File Name Size Added Link
Focus on... Ara h 1 119.65KB 22/05/2014 Download
Focus on… Ara h 1 – a Major Allergen (vicilin) from Peanut
Focus on... Peanut Allergens 317.68KB 22/05/2014 Download
Focus on... Peanut Allergens
EL-AH1_CoA 377.21KB 28/05/2014 Download
Certificate of Analysis for Ara h 1 ELISA kit
    54.156.67.122
© 2017 INDOOR Biotechnologies, Inc.      Find us on: