Blo t 5 ELISA kit (EL-BT5)

Blo t 5 ELISA kit (EL-BT5)
Product Code: EL-BT5
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring Blomia tropicalis allergen, Blo t 5.
Antibodies: 4D9/4G9
Sensitivity: 0.5ng/ml
Limit of Detection: 2ng/ml
Assay Performance: Inquire
Contents:

Monoclonal antibody 4D9, Blot t 5 Standard, Biotinylated antibody 4G9; sufficient reagents for 10 plates.

Storage: Store at 4°C.
Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6
Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine Serum Albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-BT5 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Monoclonal Antibody: 4D9 (clone 4D9 G12)
Vial: Red Cap; 100µl
Immunogen: Blo t 5
Isotype: Mouse IgG1
Specificity:

Binds to a species specific epitope on Blomia tropicalis allergen, Blo t 5.

Purification: From ascites by ammonium sulphate precipitation and by HPLC using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
Concentration:  3mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Monoclonal Antibody: 4G9 (clone 4G9 A12 D5A5)
Vial: Brown Cap; 100µl
Immunogen: Blo t 5
Isotype: Mouse IgG2A
Specificity: Binds to a species specific epitope on Blomia tropicalis allergen, Blo t 5.
Purification: From ascites by ammonium sulphate precipitation and by HPLC using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free

  Certificate of Analysis
Allergen Standard: rBlo t 5
Vial: White Cap; 400µl
Composition: Recombinant Blo t 5 prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4
Concentration: 2500ng/ml rBlo t 5
Calibration: The rBlo t 5 was expressed as a GST-fusion protein, purified over Glutathione Sepharose and further purified after trypsin cleavage by gel filtration HPLC. The protein concentration was determined by amino acid analysis.
References:

1.  Arruda LK, Rizzo MC, Chapman MD, Fernandez-Caldas E, Baggio D, Platts-Mills TAE, Naspitz CK.  Exposure and sensitization to dust mite allergens among asthmatic children in Sno Paulo, Brazil.  Clinical Exp Allergy 1991; 21:433-439.

2.  Vailes LD, Rizzo MC, Naspitz CK, Montealegre F, Fernandez-Caldas E, Platts-Mills TAE, Chapman MD, Arruda LK.  Analysis of IgE antibody responses to D. pteronyssinus and  Blomia tropicalis  using recombinant group 5 allergens.  J Allergy Clin Immunol 1996; 97:213 (Abstract).

3.  Arruda LK, Vailes LD, Platts-Mills TAE, Fernandez-Caldas E, Montealegre F, Lin KL, Chua KY, Rizzo MC, Naspitz CK, Chapman MD.  Sensitization to Blomia tropicalis in patients with asthma and identification of allergen Blo t 5.  Amer J Resp Crit Care Med 1997; 155:343-350.

(For complete reference list see EL-BT5 Certificate of Analysis)

 

File Name Size Added Link
EL-BT5_CoA 381.22KB 22/05/2014 Download
Certificate of Analysis for Blo t 5 ELISA kit
    54.82.79.109
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