Bla g 2 ELISA kit (EL-BG2)

Bla g 2 ELISA kit (EL-BG2)
Product Code: EL-BG2
Availability: In Stock
Price: $795.00
Qty:  

ELISA kit for measuring Blattella germanica allergen, Bla g 2.

Antibodies: 7C11/pAb
Sensitivity: 0.5ng/ml
Limit of Detection: 0.5ng/ml
Assay Performance: Inquire
Contents:

Monoclonal antibody 7C11, Universal Allergen Standard, Polyclonal Rabbit anti-Bla g 2; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Peroxidase conjugated Goat anti. Rabbit IgG
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-BG2 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Monoclonal Antibody: 7C11 (Clone 7C11 C2 A6)
Vial: Red Cap; 100µl
Immunogen: Bla g 2
Isotype: Mouse IgG1
Specificity: Binds to an epitope on cockroach Blattella germanica allergen, Bla g 2.
Purification: Produced in ascites and purified by ammonium sulphate precipitation and affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1mg/ml in phosphate buffered saline, pH 7.4, based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Antibody: Rabbit polyclonal antiserum
Vial: Brown Cap; 100µl
Immunogen: Recombinant Bla g 2
Specificity: The antiserum contains IgG antibodies to cockroach Blattella germanica allergen, Bla g 2 and does not react with Bla g 1.
Activity: Titrated for use in ELISA at 1/1000 dilution. Prepared in 1%BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.
   

  Certificate of Analysis
Allergen Standard: Universal Allergen Standard
Vial: White Cap; 400µl/vial
Composition: A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration:  
Universal Allergen Standard
Protein Measurement
Concentration (ng/ml)
Der p 1
Amino-acid analysis
2500
Der f 1
Amino-acid analysis
2500
Der p 2
Amino-acid analysis
1000
Fel d 1
Amino-acid analysis
1000
Can f 1
Amino-acid analysis
2500
Rat n 1
Amino-acid analysis
1000
Mus m 1
Amino-acid analysis
250
Bla g 2
Amino-acid analysis
2500
References:  
1.  Pollart SM, Smith TF, Morris E, Gelber LE, Platts-Mills TAE, Chapman MD. Environmental exposure to cockroach allergens: analysis with monoclonal Antibody based enzyme immunoassays. J Allergy Clin Immunol 87:505- 510, 1991.
2.  Gelber LE, Seltzer LH, Bouzoukis JK, Pollart SM, Chapman MD, Platts-Mills TAE. Sensitization ad exposure to indoor allergens as risk factors for asthma among patients presenting to hospital. Amer Rev Respir Dis 147:573-578, 1993.
3.  Rosenstreich DL, Eggleston P, Kattan M, Baker D, Slavin RG, Gergen P, Mitchell H, McNiff-Mortimer K, Lynn H, Ownby D, Malveaux F. The role of cockroach allergy and exposure to cockroach allergen in causing morbidity among inner-city children with asthma. New Eng J Med 336:1356-1363, 1997.
4.  Arruda LK, Vailes LD, Ferriani VP, Santos AB, Pomés A, Chapman MD. Cockroach allergens and asthma. J Allergy Clin Immunol 107:419-428, 2001.
5. Pomés A, Vailes LD, Helm RM, Chapman MD. IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1. Eur J Biochem 269:3086-3092, 2002.
6.  Nalyanya G, Gore JC, Linker HM, Schal C. German cockroach allergen levels in North Carolina schools: comparison of integrated pest management and conventional cockroach control. J Med Entomol 46:420-427, 2009.
(For complete reference list see EL-BG2 Certificate of Analysis)

 

File Name Size Added Link
EL-BG2_CoA 387.75KB 21/05/2014 Download
Certificate of Analysis for Bla g 2 ELISA kit
    54.92.141.211
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