Bla g 1 ELISA kit (EL-BG1)

Bla g 1 ELISA kit (EL-BG1)
Product Code: EL-BG1
Availability: In Stock
Price: $795.00
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ELISA kit for measuring Blattella germanica allergen, Bla g 1.
Antibodies: 10A6/pAb
Sensitivity: 0.20ng/ml
Limit of Detection: 0.80ng/ml
Assay Performance: Inquire
Contents:

Monoclonal antibody 10A6, Bla g 1 Standard, Rabbit anti-Bla g 1; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Peroxidase conjugated Goat anti. Rabbit IgG
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-BG1 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Monoclonal Antibody: 10A6 (clone 10A6 F5 E1)
Immunogen: Bla g 1
Isotype: Mouse IgG1
Specificity: Binds to a common epitope on cockroach Blattella germanica allergen Bla g 1 and Periplaneta americana allergen, Per a 1.
Purification: From ascites by ammonium sulphate precipitation and by HPLC using recombinant protein G. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1mg/ml in phosphate buffered saline, pH 7.4 Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Antibody: Polyclonal antibody raised against recombinant Bla g 1.
Specificity: The pAb contains IgG antibodies to Bla g 1 and does not react with Bla g 2.
Activity: The pAb is in phosphate buffered saline, pH 7.4, containing 1%BSA/50% glycerol. The pAb has been 0.22μm filtered and should be diluted 1/1000 for use in Bla g 1 ELISA.

  Certificate of Analysis
Allergen Standard: rBla g 1
Composition: Purified from Pichia pastoris culture by affinity chromatography and prepared in 1% BSA, 50% glycerol/PBS, pH 7.4.

Concentration:

1000ng/ml

Calibration:

The concentration of the purified recombinant Bla g 1 was determined by amino acid analysis. There are no national or international reference standards for cockroach allergens.

Notes:

Bla g 1 measurements were previously reported in arbitrary units (U/ml).  Studies carried out with rBla g 1, Lot 36019 have determined that 1 Unit is ~100ng Bla g 1 protein.

References:  
1. Pollart SM, Smith TF, Morris E, Gelber LE, Platts-Mills TAE, Chapman MD. Environmental exposure to cockroach allergens: analysis with monoclonal Antibody based enzyme immunoassays. J Allergy Clin Immunol 87:505-510, 1991.
2. Gelber LE, Seltzer LH, Bouzoukis JK, Pollart SM, Chapman MD, Platts-Mills TAE. Sensitization ad exposure to indoor allergens as risk factors for asthma among patients presenting to hospital. Amer Rev Respir Dis 147:573-578, 1993.
3. Rosenstreich DL, Eggleston P, Kattan M, Baker D, Slavin RG, Gergen P, Mitchell H, McNiff-Mortimer K, Lynn H, Ownby D, Malveaux F. The role of cockroach allergy and exposure to cockroach allergen in causing morbidity among inner-city children with asthma. New Eng J Med 336:1356-1363, 1997.
4. Arruda LK, Vailes LD, Ferriani VP, Santos AB, Pomés A, Chapman MD. Cockroach allergens and asthma. J Allergy Clin Immunol 107:419-428, 2001.
5. Pomés A, Vailes LD, Helm RM, Chapman MD. IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1. Eur J Biochem 269:3086-3092, 2002.
6. Nalyanya G, Gore JC, Linker HM, Schal C. German cockroach allergen levels in North Carolina schools: comparison of integrated pest management and conventional cockroach control. J Med Entomol 46:420-427, 2009.
(For complete reference list see EL-BG1 Certificate of Analysis)

 

File Name Size Added Link
EL-BG1_CoA 382.89KB 28/05/2014 Download
Certificate of Analysis for Bla g 1 ELISA kit
    54.87.65.140
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