Asp f 1 ELISA kit (EL-AF1)

Asp f 1 ELISA kit (EL-AF1)
Product Code: EL-AF1
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring Aspergillus fumigatus allergen, Asp f 1.
Sensitivity: 0.08ng/ml
Limit of Detection: 0.32ng/ml
Assay Performance: Inquire
Contents:

Monoclonal antibody 4A6, Asp r 1 Standard, Rabbit anti-Asp f 1; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C.
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Peroxidase conjugated Goat anti. Rabbit IgG
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-AF1 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Monoclonal Antibody: 4A6
Vial: Red Cap; 100µl
Immunogen: Asp f 1
Isotype: Mouse IgG1
Specificity: Binds to a common epitope on Aspergillus fumigatus allergen, Asp f 1.
Purification: Produced in tissue culture and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
Concentration: 2mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Antibody: Polyclonal antibody raised against partially purified Asp f 1 (1,2).
Vial: Brown Cap; 100µl
Specificity: The pAb contains IgG antibodies to Asp f 1 as well as IgG antibodies to other Aspergillus allergens.
Activity: The pAb is in phosphate buffered saline containing 1%BSA 50%glycerol PBS, pH 7.4. The pAb has been 0.22µm filtered and should be diluted 1/1000 for use in Asp f 1 ELISA.

  Certificate of Analysis
Allergen Standard: nAsp r 1
Vial: White Cap; 400µl
Composition: Naturally purified Asp r 1 prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4
Concentration: 400ng/ml Asp r 1

Concentration:  

The Asp f 1 concentration was determined by amino acid analysis.  Asp f 1 and Asp r 1 (mitogillin) are 95% homologous (differing by 4 amino acid residues) and are antigenically and functionally indistinguishable (1).
References:  
1. Arruda, LK, Platts-Mills, TAE, Fox, JW and Chapman, MD (1990). Aspergillus fumigatus allergen I, a major IgE binding protein, is a member of the mitogillin  family of cytotoxins. J. Exp. Med., 172:1529-1532. 
 
2. Arruda LK, Platts-Mills TAE, Longbottom JL, Chapman MD (1992). Aspergillus fumigatus: Identification of 16kd, 18kd and 45kd antigens recognized by human  IgG and IgE antibodies and murine monoclonal antibodies. J. Allergy Clin. Immunol. 89:1166-1176. 
 
3. Arruda LK, Mann BJ, Chapman MD (1992). Selective expression of a major allergen and cytotoxin, Asp f 1, in Aspergillus fumigatus. J. Immunol. 149:3354- 3359. 
 
4. Sporik RB, Arruda LK, Woodfolk J, Chapman MD, Platts-Mills TAE (1993).  Environmental exposure to Aspergillus fumigatus allergen 1 (Asp f 1). Clin. Exp.  Allergy 23:326-331. 
 
5. El-Dahr JM, Fink R, Selden R, Arruda LK, Platts-Mills TAE, Heymann PW (1994). Development of immune responses to Aspergillus at an early age in children with cystic fibrosis. Am. J.
 Respir. Crit. Care Med. 150:1513-1518. 
 
6. Vailes LD, Sridhara S, Cromwell O, Weber B, Breitenbach M, Chapman MD (2001). Quantitation of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products. J. Allergy Clin. Immunol. 107:641-646. 
 
7. Chapman MD (2006). Challenges associated with indoor moulds: Health effects, immune response and exposure assessment. Medical Mycology. 44:S29-S32.
(For complete reference list see EL-AF1 Certificate of Analysis)

 

File Name Size Added Link
EL-AF1_CoA 379KB 28/05/2014 Download
Certificate of Analysis for Asp f 1 ELISA kit
    54.197.130.93
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