Amb a 1 ELISA kit (EL-AM1)

Amb a 1 ELISA kit (EL-AM1)
Product Code: EL-AM1
Availability: Out Of Stock
Price: $795.00
Qty:  
ELISA kit for measuring Short Ragweed (Ambrosia artemisiifolia) allergen, Amb a 1.
Sensitivity: 0.0005U/ml
Limit of Detection: 0.002U/ml
Assay Performance: Inquire
Contents:

Rabbit anti. Amb a 1, Amb a 1 Allergen Standard, Biotinylated Rabbit anti. Amb a 1; sufficient reagents for 10 plates.

Storage: Store at 4°C.
Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6
Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine Serum Albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-AM1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Antibody: Rabbit polyclonal IgG antibodies raised against short ragweed allergen, Amb a 1 and purified by HPLC.
Vial: Red Cap; 100µl
Immunogen: Amb a 1
Isotype: Polyclonal IgG antibodies (isotypes not determined).
Specificity: Binds to Ambrosia artemisiifolia allergen, Amb a 1, in short ragweed pollen extracts.  Limited cross-reactivity with grass pollen extracts.
Purification: By HPLC using recombinant protein G.  Single heavy and light chain bands on SDS-PAGE.
Concentration: 2mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for IgG (1.42=1mg/ml), 0.22µm filtered,preservative free.

Monoclonal Antibody: Rabbit polyclonal IgG antibodies raised against short ragweed allergen, Amb a 1.
Vial: Brown Cap; 100µl
Immunogen: Amb a 1
Isotype: Polyclonal IgG antibodies (isotypes not determined).
Specificity: Binds to an epitope on natural ragweed pollen allergen, Amb a 1.
Purification: By affinity chromatography using ragweed antigen.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in PBS, 0.22µm filtered, preservative free.

Allergen Standard: Amb a 1 Standard
Vial: White Caps; 400µl
Composition: Short Ragweed Extract prepared in 1% BSA/50% glycerol/PBS, 0.22µm filtered, preservative free.
Concentration: 2.5U/ml Amb a 1
Calibration: Sub-standardized against the U.S. Food and Drug Administration radial immunodiffusion reference for Amb a 1, C14-Ras, which contains 30 Units Amb a 1/ml.
References:  
(For complete reference list see EL-AM1 Certificate of Analysis)

 

File Name Size Added Link
EL-AM1-CoA 373.71KB 28/05/2014 Download
Certificate of Analysis for Amb a 1 ELISA kit
    54.82.79.109
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