Asp f 1 ELISA 2.0 kit - Single plate (EPC-AF1-1)

Asp f 1 ELISA 2.0 kit - Single plate (EPC-AF1-1)
Product Code: EPC-AF1-1
Availability: In Stock
Price: $395.00

ELISA 2.0 kit for measuring Aspergillus fumigatus allergen, Asp f 1.

Antibodies: 4A6/pAb
Standard Range: 40-0.08ng/mL
Limit of Detection: 0.32ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents: Plate: Pre-coated with anti-Asp f 1 monoclonal antibody 4A6
Vial 1: (white top) nAsp f 1 allergen standard
Concentration: 400ng/ml
Vial 2: (brown) Rabbit anti-Asp f 1
Vial 3: (red top) Peroxidase conjugated Goat anti. Rabbit IgG
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: 6 months from date of receipt
Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-AF1-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 4A6 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 4A6
Immunogen: Asp f 1
Isotype: Mouse IgG1
Specificity: Binds to a common epitope on Aspergillus fumigatus allergen, Asp f 1.
Purification: Produced in tissue culture and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.

Detection Antibody: Polyclonal antibody raised against partially purified Asp f 1 (1,2).
Vial: Brown Cap; 100µl
Specificity: The pAb contains IgG antibodies to Asp f 1 as well as IgG antibodies to other Aspergillus allergens.
Activity: The pAb is in phosphate buffered saline containing 1%BSA 50%glycerol PBS, pH 7.4. The pAb has been 0.22µm filtered and should be diluted 1/1000 for use in Asp f 1 ELISA.

Allergen Standard: Naturally purified Asp r 1 prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4
Concentration: 400ng/mL

The Asp f 1 concentration was determined by amino acid analysis.  Asp f 1 and Asp r 1 (mitogillin) are 95% homologous (differing by 4 amino acid residues) and are antigenically and functionally indistinguishable (1).

1. Arruda, LK, Platts-Mills, TAE, Fox, JW and Chapman, MD (1990). Aspergillus fumigatus allergen I, a major IgE binding protein, is a member of the mitogillin  family of cytotoxins. J. Exp. Med., 172:1529-1532. 
2. Arruda LK, Platts-Mills TAE, Longbottom JL, Chapman MD (1992). Aspergillus fumigatus: Identification of 16kd, 18kd and 45kd antigens recognized by human  IgG and IgE antibodies and murine monoclonal antibodies. J. Allergy Clin. Immunol. 89:1166-1176. 
3. Arruda LK, Mann BJ, Chapman MD (1992). Selective expression of a major allergen and cytotoxin, Asp f 1, in Aspergillus fumigatus. J. Immunol. 149:3354- 3359. 
4. Sporik RB, Arruda LK, Woodfolk J, Chapman MD, Platts-Mills TAE (1993).  Environmental exposure to Aspergillus fumigatus allergen 1 (Asp f 1). Clin. Exp.  Allergy 23:326-331. 
5. El-Dahr JM, Fink R, Selden R, Arruda LK, Platts-Mills TAE, Heymann PW (1994). Development of immune responses to Aspergillus at an early age in children with cystic fibrosis. Am. J.
 Respir. Crit. Care Med. 150:1513-1518. 
6. Vailes LD, Sridhara S, Cromwell O, Weber B, Breitenbach M, Chapman MD (2001). Quantitation of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products. J. Allergy Clin. Immunol. 107:641-646. 
7. Chapman MD (2006). Challenges associated with indoor moulds: Health effects, immune response and exposure assessment. Medical Mycology. 44:S29-S32.
(For complete reference list see EPC-AF1 Certificate of Analysis)


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