Ara h 6 ELISA 2.0 kit - Single plate (EPC-AH6-1)

Ara h 6 ELISA 2.0 kit - Single plate (EPC-AH6-1)
Product Code: EPC-AH6-1
Availability: In Stock
Price: $395.00
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ELISA 2.0 kit for measuring Arachis hypogaea allergen, Ara h 6.

Antibodies: 3B8/3E12
Standard Range: 25-0.05ng/mL
Limit of Detection: 0.2ng/mL
Background: OD<0.08ng/ml at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with anti-Ara h 6 monoclonal antibody 3B8
Vial 1: (white top) Ara h 6 allergen standard
Concentration: 250 ng/ml
Vial 2: (brown) anti-Ara h 6 antibody 3E12
Vial 3: (red top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: 6 months from date of receipt
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-AH6-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 3B8 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 3B8
Immunogen: Ara h 6
Isotype: Mouse IgG1
Specificity: Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 6.
Purification: Produced in ascites and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: 3E12
Immunogen: Ara h 6
Isotype Mouse IgG1
Specificity: Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 6.
Activity: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Purified natural Ara h 6 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration/Calibration: 250ng/mL (based on amino acid analysis)
References:  
1.  Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6.
2.  Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.
 

 

Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 6.
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