Alt a 1 ELISA 2.0 kit - Single plate pack (EPC-AA1-1)

Alt a 1 ELISA 2.0 kit - Single plate pack (EPC-AA1-1)
Product Code: EPC-AA1-1
Availability: In Stock
Price: $395.00
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ELISA 2.0 kit for measuring Alternaria alternata allergen, Alt a 1.
Antibodies: 2C10/3B6
Standard Range: 25-0.05 ng/ml
Limit of Detection: 0.05ng/ml
Background OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents: Plate: Pre-coated with anti-Alt a 1 monoclonal antibody 2C10
Vial 1: (white top) Alt a 1 allergen standard
Concentration: 250 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 3B6
Vial 3: (red top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: 6 months from date of receipt
Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources: EPC-AA1-1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 2C10 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 2C10 (clone 2C10 G4)
Immunogen: Alt a 1
Isotype: Mouse IgG
Specificity: Binds to a species specific epitope on Alternaria alternata allergen, Alt a 1.
Purification: Produced in vitro cell culture and purified by chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: 3B6 (clone 3B6 H9)
Immunogen: rAlt a 1
Isotype: Mouse IgG
Specificity: Binds to a species specific epitope on Alternaria alternata allergen, Alt a 1.
Purification: Produced in vitro cell culture and purified by chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Recombinant Alt a 1 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration: 250 ng/mL (The rAlt a 1 was purified from Pichia pastoris by size exclusion HPLC. The protein content was determined by amino acid analysis.)

References:

  1. Vailes L, Sridhara S, Cromwell O, Weber B, Breitenbach M, Chapman M. Quantification of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products. J Allergy Clin Immunol 2001;107(4):641-6.
  2. Unger A, Stoger P, Simon-Nobbe B, Susani M, Crameri R, Ebner C et al. Clinical testing of recombinant allergens of the mold Alternaria alternata. Int Arch Allergy Immunol 1999;118(2-4):220-1.
  3. Asturias JA, Ibarrola I, Ferrer A, Andreu C, Lopez-Pascual E, Quiralte J et al. Diagnosis of Alternaria alternata sensitization with natural and recombinant Alt a 1 allergens. J Allergy Clin Immunol 2005;115(6):1210-7.
  4. Chruszcz M, Chapman MD, Osinski T, Solberg R, Demas M, Porebski PJ et al. Alternaria alternata allergen Alt a 1: A unique beta-barrel protein dimer found exclusively in fungi. J Allergy Clin Immunol 2012;130(1):241-7.
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