Can f 1 ELISA 2.0 kit - Single plate (EPC-CF1-1)

Can f 1 ELISA 2.0 kit - Single plate (EPC-CF1-1)
Product Code: EPC-CF1-1
Availability: In Stock
Price: $395.00
Qty:  

ELISA 2.0 kit for measuring Canis familiaris allergen, Can f 1.

Antibodies: 10D4/pAb
Standard Range: 50-0.1 ng/mL
Limit of Detection: 0.39ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with anti-Can f 1 monoclonal antibody 10D4
Vial 1: (white top) Can f 1 allergen standard
Concentration: 500 ng/ml
Vial 2: (brown) Rabbit anti Can f 1 antibody
Vial 3: (red top) Peroxidase-conjugated Goat Anti-Rabbit
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: 6 months from date of receipt
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources: EPC-CF1-1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 10D4 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 10D4 (clone 10D4 C4 A10)
Immunogen: Can f 1
Isotype: Mouse IgG2ak
Specificity: Binds to an epitope on dog Canis familiaris allergen, Can f 1.
Purification: Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: Rabbit polyclonal antiserum
Immunogen: Dog fur extract
Isotype Multiple
Specificity: The antiserum contains IgG antibodies to dog
Canis familiaris allergen, Can f 1, as well as IgG antibodies to other dog allergens..
Activity: Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Purified natural Can f 1 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration/Calibration: 500 ng/mL (based on amino acid analysis)
References:  
1.  Larsen JN, Ford A, Gjesing B, et al. The collaborative study of the international standard of dog, Canis domesticus, hair/dander extract. J Allergy Clin Immunol 1988; 82:318-330.
2.  DeGroot H, Goei K, Van Sicter P, et al. Affinity purification of a major and minor allergen from dog extract: Serologic activity of affinity-purified Can f 1 and Can f 1-depleted extract. J Allergy Clin Immunol 1991; 87:1056-61.
3.  Schou C, Hansen G, Linter T, et al. Assay for the major dog allergen, Can f 1 investigation of house dust samples and commercial dog extracts. J Allergy Clin Immunol 1992; 88:847-53.
4.  Ingram JM, Sporik R, Rose G, Honsiger R, Chapman MD, Platts-Mills TAE. Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens: Relationship to sensitization and asthma among children living in Los Alamos, New Mexico. J Allergy Clin Immunol 1995; 96:449-456.
5.  Sporik RB, Ingram JM, Price W, Sussman JH, Honsiger RW, Platts-Mills TAE. Association of asthma with serum IgE and skin test reactivity to allergens among children living at high altitude: Tickling the dragon’s breath. Amer J Resp Crit Care Med 1995;151:1388-1392.
 

 

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EPC-CF1-X_CoA 458.86KB 19/02/2015 Download
Certificate of Analysis for Can f 1 ELISA 2.0 kit
    54.196.53.39
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