Der p 1 ELISA kit (EL-DP1)

Der p 1 ELISA kit (EL-DP1)
Product Code: EL-DP1
Availability: In Stock
Price: $795.00
Qty:  

ELISA kit for measuring D. pteronyssinus allergen, Der p 1.

Antibodies: 5H8/4C1
Sensitivity: 0.5ng/ml
Limit of Detection: 2ng/ml
Assay Performance: Intra-assay CV% of 6.4; Inter-assay CV% of 15
Contents:

Monoclonal antibody 5H8, Universal Allergen Standard, Biotinylated antibody 4C1; sufficient reagents for 10 plates.

Storage: Store at 4°C.
Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6
Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine Serum Albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-DP1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Monoclonal Antibody: 5H8 (clone 5H8 C12 D8)
Vial: Red Cap; 100µl
Immunogen: Der p 1
Isotype: Mouse IgG2A
Specificity: Binds to an epitope present on Dermatophagoides pteronyssinus allergen, Der p 1.
Purification: Produced in tissue culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Concentration: 2.0mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for IgG (1.42=1mg/ml), 0.22µm filtered, preservative free.

Monoclonal Antibody: 4C1 (clone 4C1 B8 3F8)
Vial: Brown Cap; 100µl
Immunogen: Der f 1
Isotype: Mouse IgG1
Specificity: Binds to an epitope on dust mite Dermatophagoides Group 1 allergens (Der f 1, Der p 1, Der m 1, Eur m 1).
Purification: Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

Allergen Standard: Universal Allergen Standard
Vial: White Cap; 400µl
Composition: A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration:  
Universal Allergen Standard
Protein Measurement
Concentration (ng/ml)
Der p 1
Amino-acid analysis
2500
Der f 1
Amino-acid analysis
2500
Der p 2
Amino-acid analysis
1000
Fel d 1
Amino-acid analysis
1000
Can f 1
Amino-acid analysis
2500
Rat n 1
Amino-acid analysis
1000
Mus m 1
Amino-acid analysis
250
Bla g 2
Amino-acid analysis
2500
References:    
1. Luczynska CM, Arruda LK, Platts-Mills TA, Miller JD, Lopez M, Chapman MD. A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p I and Der f I. J Immunol Methods 1989; 118(2):227-235.  
2. Custovic A, Taggart SC, Francis HC, Chapman MD, Woodcock A. Exposure to house dust mite allergens and the clinical activity of asthma. J Allergy Clin Immunol 1996; 98(1):64-72.
3. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24.
4. Sawyer G, Kemp T, Shaw R et al. Biologic pollution in infant bedding in New Zealand: high allergen exposure during a vulnerable period. J Allergy Clin Immunol 1998; 102(5):765-770.
5. Arbes SJ, Jr., Cohn RD, Yin M et al. House dust mite allergen in US beds: results from the First National Survey of Lead and Allergens in Housing. J Allergy Clin Immunol 2003; 111(2):408-414.
6. Gruchalla RS, Pongracic J, Plaut M et al. Inner City Asthma Study: relationships among sensitivity, allergen exposure, and asthma morbidity. J Allergy Clin Immunol 2005; 115(3):478-485.
7. Zock JP, Heinrich J, Jarvis D et al. Distribution and determinants of house dust mite allergens in Europe: the European Community Respiratory Health Survey II. J Allergy Clin Immunol 2006; 118(3):682-690.
8. Jarvis D, Zock JP, Heinrich J et al. Cat and dust mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. J Allergy Clin Immunol 2007; 119:697-704.
9. Earle CD, King EM, Tsay A et al. High-throughput fluorescent multiplex array for indoor allergen exposure assessment. J Allergy Clin Immunol 2007; 119(2):428-433.
(For complete reference list see EL-DP1 Certificate of Analysis)

 

File Name Size Added Link
EL-DP1_CoA 436.07KB 28/05/2014 Download
Certificate of Analysis for Der p 1 ELISA kit
    54.196.107.247
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