Der f 1 ELISA 2.0 kit - Single plate (EPC-DF1-1)

Der f 1 ELISA 2.0 kit - Single plate (EPC-DF1-1)
Product Code: EPC-DF1-1
Availability: In Stock
Price: $395.00
Qty:  
ELISA 2.0 kit for measuring D. farinae allergen, Der f 1.
Antibodies: 6A8/4C1
Standard Range: 50-0.1 ng/ml
Limit of Detection: 0.19ng/ml
Background OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents: Plate: Pre-coated with anti-Der f 1 monoclonal antibody 6A8
Vial 1: (white top) Der f 1 allergen standard
Concentration: 500 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 4C1
Vial 3: (red top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: 6 months from date of receipt
Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources: EPC-DF1-1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 6A8 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 6A8 (clone 6A8 B10 D12)
Immunogen: Der f 1
Isotype: Mouse IgG1
Specificity: Binds to a species specific epitope on dust mite Dermatophagoides farinae allergen, Der f 1.
Purification: Produced in tissue culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: 4C1 (clone 4C1 B8 3F8)
Immunogen: Der f 1
Isotype: Mouse IgG1
Specificity: Binds to a common epitope present on mite Dermatophagoides Group 1 allergens (Der f 1, Der p 1, Der m 1, Eur m 1).
Purification: Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Purified natural Der f 1 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration: 500 ng/mL (based on amino acid analysis)

References:

1.   Chapman, M.D., Sutherland, W.M., and Platts-Mills, T.A.E. (1984).  Recognition of two Dermatophagoides pteronyssinus specific epitopes on Antigen P1 using  monoclonal antibodies:  Binding to each epitope can be inhibited be sera from mite allergic patients.  J. Immunol. 133:2488-2495.

2.  Ford, A.W., Rawle, F.C., Lind, P., Spieksma, F.Th.M., Lowenstein, H. and Platts-Mills, T.A.E. (1985). Standardization of Dermatophagoides pteronyssinus: Assessment of potency and allergen content in ten coded extracts. Int. Archs. Allergy Appl. Immunol. 76:58-68.

3.  Heymann, P.W., Chapman, M.D. and Platts-Mills, T.A.E. (1986). Antigen Der f 1 from the dust mite Dermatophagoides farinae: Structural comparison with Der p 1 from D. pteronyssinus and epitope specificity of murine IgG and human IgE antibodies. J. Immunol. 137:2841-2847.

4.   Chapman, M.D., Heyman, P.W., Wilkins, S.R., Brown, M.J., and Platts-Mills, T.A.E. (1987). Monoclonal immunoassays for major dust mite (Dermatophagoides) allergens, Der p 1 and Der f 1, and quantitative analysis of the allergen content of mite and house dust extracts. J. Allergy Clin. Immunol. 80:184-194.

5.  Platts-Mills, T.A.E. and Chapman, M.D. (1987). Dust mites: Immunology, allergic disease and environmental control. J. Allergy Clin. Immunol. 80:755-775

6.  Chapman, M.D., Heymann, P.W., and Platts-Mills, T.A.E. (1987). Epitope mapping of two major inhalant allergens, Der p 1 and Der f 1, from mites of the genus Dermatophagoides. J. Immunol. 139: 1479-1484.

7.  Chapman, M.D. (1988). Allergen specific monoclonal antibodies: New tools for the management of allerigic disease. Allergy 43 (5):7-14.

8.  Luczynska, C.M., Arruda, L.K., Platts-Mills, T.A.E., Miller, J.D., Lopez, M., and Chapman, M.D. (1989). A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p 1 and Der f 1. J. Immunol. Meths. 118:227-235.

9.  Platts-Mills T.A.E., Thomas W.R., Aalberse R.C., Vervloet D. and Chapman M.D. (1992). Dust mite allergens and asthma: Report of a second international workshop. J Allergy Clin Immunol 89:1046-1060.

10.  Platts-Mills T.A.E., Vervloet D., Thomas W.R., Aalberse R.C., Chapman M.D. Indoor Allergens and Asthma: Report of the third international workshop. J Allergy Clin Immunol, 1997; 100:S1-S24.

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Certificate of Analysis for Der f 1 ELISA 2.0 kit
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