Guinea Pig Urinary Protein ELISA kit (EL-GPUP)

Guinea Pig Urinary Protein ELISA kit (EL-GPUP)
Product Code: EL-GPUP
Availability: In Stock
Price: $795.00
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ELISA kit for measuring Guinea Pig Urinary Protein allergen, GPUP.
Antibodies: pAb/pAb
Sensitivity: 0.5ng/ml
Limit of Detection: 7.8ng/ml
Assay Performance: Inquire
Contents:

Rabbit polyclonal antiserum, nGPUP Standard, Rabbit polyclonal antiserum; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Peroxidase conjugated Goat anti. Rabbit IgG
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-GPUP Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Monoclonal Antibody: Rabbit polyclonal antiserum
Immunogen: Guinea Pig Urinary Protein
Isotype: Multiple
Specificity: Binds to guinea pig urinary proteins. Reacts with guinea pig epithelial and hair extracts. Does not recognize rat or mouse urinary proteins; does not react with rabbit or hamster epithelial and hair extracts.
Purification: Affinity chromatography using recombinant Protein G.
Concentration: 1-2mg/ml in phosphate buffered saline, pH 7.4 Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Antibody: Rabbit polyclonal antiserum
Specificity: Binds to guinea pig urinary proteins. Reacts with guinea pig epithelial and hair extracts. Does not recognize rat or mouse urinary proteins; does not react with rabbit or hamster epithelial and hair extracts.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1,000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4. 0.22μm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: nGPUP
Composition: Purified natural guinea pig urinary protein prepared in
1% BSA/50% glycerol/PBS, pH 7.4.

Concentration:

10,000ng/mL as determined by Advanced Protein Assay

References:  
1. Hilger C, Swiontek K, Kler S, Diederich C, Lehners C, Vogel L, Vieths S, Hentges F. Evaluation of two new recombinant guinea-pig lipocalins, Cav p 2 and Cav p 3, in the diagnosis of guinea-pig allergy. Clin Exp
Allergy. 2011 Jun; 41(6):899-908.
 

2. Ferraz E, Arruda LK, Bagatin E, Martinez EZ, Cetlin AA, Simoneti CS, Freitas AS, Martinez JA, Borges MC, Vianna EO. Laboratory animals and respiratory allergies: the prevalence of allergies among laboratory animal workers and the need for prophylaxis. Clinics (Sao Paulo). 2013 Jun; 68(6): 750–759. 

(For complete reference list see EL-GPUP Certificate of Analysis)

 

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