Rat n 1 ELISA kit (EL-RN1)

Rat n 1 ELISA kit (EL-RN1)
Product Code: EL-RN1
Availability: In Stock
Price: $795.00
Qty:  

ELISA kit for measuring Rat Urinary Protein (RUP) allergen, Rat n 1.

Antibodies: RUP-6/RUP-1
Sensitivity: 0.2ng/ml
Limit of Detection: 0.8ng/ml
Assay Performance: Inquire
Contents:

Monoclonal antibody RUP-6, Universal Allergen Standard, Biotinylated antibody RUP-1; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-RN1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Monoclonal Antibody: RUP-6
Vial: Red Cap; 100µl
Immunogen: Rat n 1
Isotype: Mouse IgG1
Specificity: Binds to an epitope on rat Rattus norvegicus urinary allergen, Rat n 1.
Purification: Produced in cell culture and purified by affinity chromatography using Protein G.
Concentration: 1.0mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, 0.02% sodium azide.

  Certificate of Analysis
Biotinylated Antibody: RUP-1
Vial: Brown Cap; 100µl
Immunogen: Rat n 1
Isotype: Mouse IgG1
Specificity:

Binds to an epitope on rat Rattus norvegicus urinary allergen, Rat n 1.

Purification: Produced in cell culture and purified by affinity chromatography using Protein G.
Concentration: 1.0mg/ml in phosphate buffered saline. Based on A280 for IgG (1.42= 1mg/ml), 0.22µm filtered, 0.02% sodium azide.
Biontinylation: Biotinylated and titrated for use in ELISA at 1/1000
dilution.

  Certificate of Analysis
Allergen Standard: Universal Allergen Standard
Vial: White Cap; 400µl/vial
Composition: A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration:  
Universal Allergen Standard
Protein Measurement
Concentration (ng/ml)
Der p 1
Amino-acid analysis
2500
Der f 1
Amino-acid analysis
2500
Der p 2
Amino-acid analysis
1000
Fel d 1
Amino-acid analysis
1000
Can f 1
Amino-acid analysis
2500
Rat n 1
Amino-acid analysis
1000
Mus m 1
Amino-acid analysis
250
Bla g 2
Amino-acid analysis
2500

References:

1.  Renstrom A, Larsson PH, Malmberg P, Bayard C. A new amplified monoclonal rat allergen assay used for evaluation of ventilation improvements in animal rooms. J Allergy Clin Immunol. 1997 Nov;100(5):649-55.

2.  Renstrom A, Gordon S, Larsson PH, Tee RD, Newman Taylor AJ, Malmberg P. Comparison of a radioallergosorbent (RAST) inhibition method and a monoclonal enzyme linked immunosorbent assay (ELISA) for aeroallergen measurement. Clin Exp Allergy. 1997 Nov;27(11):1314-21.

(For complete reference list see EL-RN1 Certificate of Analysis)

 

File Name Size Added Link
EL-RN1_CoA 384.31KB 21/05/2014 Download
Certificate of Analysis for Rat n 1 ELISA kit
    54.167.218.210
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