Fel d 4 ELISA kit (EL-FD4)

Fel d 4 ELISA kit (EL-FD4)
Product Code: EL-FD4
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring Felis domesticus allergen, Fel d 4.
Antibodies: 5F3/pAb
Sensitivity: 0.02ng/ml
Limit of Detection: 0.08ng/ml
Assay Performance: Please inquire
Contents:

Monoclonal antibody 5F3, Fel d 4 Standard, Rabbit anti-Fel d 4; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Peroxidase conjugated Goat anti. Rabbit IgG
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources:

EL-FD4 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Monoclonal Antibody: 5F3
Vial: Red Cap; 100µl
Immunogen: Fel d 4
Isotype: Mouse IgG1
Specificity:

Binds to an epitope on cat Felis domesticus allergen, Fel d 4.

Purification: Produced in vitro and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1.19 mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Antibody: Rabbit polyclonal antiserum
Vial: Brown Cap; 100µl
Immunogen: Fel d 4
Isotype: Multiple
Specificity: The antiserum contains IgG antibodies to cat Felis domesticus allergen, as well as IgG antibodies to other cat allergens.
Activity: Titrated for use in ELISA for Fel d 4 allergen at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: rFel d 4
Vial: White Cap; 400µl
Composition: Recombinant Fel d 4 prepared in 1% BSA/50% glycerol/PBS, pH 7.4
Concentration: 100ng/ml
Calibration: The concentration of the purified recombinant Fel d 4 was determined by amino acid analysis. There are no national or international reference standards for cat allergens.

References:

1.  Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24.

2.  Platts-Mills TA, Vaughan J, Squillace S, Woodfolk J, Sporik R. Sensitisation, asthma, and a modified Th2 response in children exposed to cat allergen: a population-based cross-sectional study. Lancet 2001; 357(9258):752-756.

3.  Heinrich J, Bedada GB, Zock JP, Chinn S, Norback D, Oliveri M et al. Cat allergen level: its determinants and relationships to specific IgE to cat across European centers. J Allergy Clin Immunol 2006; 118(3):674-681

4.  Jarvis D, Zock JP, Heinrich J, Svanes C, Verlato G, Olivieri M et al. Cat and dust mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. J Allergy Clin Immunol. 2007 Mar; 119(3):697-704.

(For complete reference list see EL-FD4 Certificate of Analysis)

 

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