Fel d 1 ELISA 2.0 kit - Single plate (EPC-FD1-1)

Fel d 1 ELISA 2.0 kit - Single plate (EPC-FD1-1)
Product Code: EPC-FD1-1
Availability: In Stock
Price: $395.00
Qty:  
ELISA 2.0 kit for measuring Felis domesticus allergen, Fel d 1.
Antibodies: 6F9/3E4
Standard Range: 25-0.05ng/mL
Limit of Detection: 0.1ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with anti-Fel d 1 monoclonal antibody 6F9
Vial 1: (white top) Fel d 1 allergen standard
Concentration: 250ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 3E4
Vial 3: (red top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C
Expiry: 6 months from date of receipt
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-FD1-1 Certificate of Analysis

Focus on... Fel d 1

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 5H8 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 6F9 (clone 6F9 A4 H1)
Immunogen: Fel d 1
Isotype: Mouse IgG1
Specificity:

Binds to an epitope on cat Felis domesticus allergen, Fel d 1, Chain 1.

Purification: Produced in tissue culture and purified by affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: 3E4 (clone 3E4 C4 C10)
Immunogen: Fel d 1
Isotype: Mouse IgG1
Specificity: Binds to an epitope on cat Felis domesticus allergen, Fel d 1, Chain 1.
Purification: Produced in ascites and purified by ammonium sulphate precipitation and HPLC using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA for Fel d 1 allergen at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Purified natural Fel d 1 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration: 250ng/mL (based on amino acid analysis)

References:

1.  Chapman MD, Aalberse RC, Brown MJ, Platts-Mills TA. Monoclonal antibodies to the major feline allergen Fel d 1. 11. Single step affinity purification of Fel d 1, N terminal sequence analysis, and development of a sensitive two-site immunoassay to assess Fel d 1 exposure. J Immunol 1988; 140(3):812-818.

2.  de Blay F, Chapman MD, Platts-Mills TA. Airborne cat allergen (Fel d 1). Environmental control with the cat in situ. Am Rev Respir Dis 1991; 143:1334-39.

3.  Ingram JM, Sporik R, Rose G, Honsinger R, Chapman MD, Platts-Mills TA. Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens: relation to sensitization and asthma among children living in Los Alamos, New Mexico. J Allergy Clin Immunol 1995; 96(4):449-456.

4.  Patchett K, Lewis S, Crane J, Fitzharris P. Cat Allergen (Fel d 1) levels on school children’s clothing and in primary school classrooms in Wellington, New Zealand. J Allergy Clin Immunol 1997; 100(6 Pt ):755-759

5.  Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24.

6.  Platts-Mills TA, Vaughan J, Squillace S, Woodfolk J, Sporik R. Sensitisation, asthma, and a modified Th2 response in children exposed to cat allergen: a population-based cross-sectional study. Lancet 2001; 357(9258):752-756.

7.  Arbes SJ, Jr.,Cohn RD, Yin M, Muilenberg ML, Friedman W, Zeldin DC. Dog allergen (Can f 1) and cat allergen (Fel d 1) in US homes: results from the National Survey of Lead and Allergens in Housing. J Allergy Clin Immunol 2004; 114(1):111- 117.

8.  Heinrich J, Bedada GB, Zock JP, Chinn S, Norback D, Oliveri M et al. Cat allergen level: its determinants and relationships to specific IgE to cat across European centers. J Allergy Clin Immunol 2006; 118(3):674-681

9.  Jarvis D, Zock JP, Heinrich J, Svanes C, Verlato G, Olivieri M et al. Cat and dust mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. J Allergy Clin Immunol. 2007 Mar; 119(3):697-704.

 

 

File Name Size Added Link
Focus on... Fel d 1 88.61KB 22/05/2014 Download
Focus on... Fel d 1 - The most potent allergen in cat dander
EPC-FD1-X_CoA 509.93KB 19/02/2015 Download
Certificate of Analysis for Fel d 1 ELISA 2.0 kit
    54.156.67.122
© 2017 INDOOR Biotechnologies, Inc.      Find us on: