Fel d 1 ELISA kit (EL-FD1)

Fel d 1 ELISA kit (EL-FD1)
Product Code: EL-FD1
Availability: In Stock
Price: $795.00
Qty:  
ELISA kit for measuring Felis domesticus allergen, Fel d 1.
Antibodies: 6F9/3E4
Sensitivity: 0.2ng/ml
Limit of Detection: 0.8ng/ml
Assay Performance: Intra-assay CV% of 8.3; Inter-assay CV% of 19
Contents:

Monoclonal antibody 6F9, Universal Allergen Standard, Biotinylated antibody 3E4; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources:

EL-FD1 Certificate of Analysis

Focus on... Fel d 1

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Monoclonal Antibody: 6F9 (clone 6F9 A4 H1)
Vial: Red Cap; 100µl
Immunogen: Fel d 1
Isotype: Mouse IgG1
Specificity:

Binds to an epitope on cat Felis domesticus allergen, Fel d 1, Chain 1.

Purification: Produced in tissue culture and purified by affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1.0 mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Monoclonal Antibody: 3E4 (clone 3E4 C4 C10)
Vial: Brown Cap; 100µl
Immunogen: Fel d 1
Isotype: Mouse IgG1
Specificity: Binds to an epitope on cat Felis domesticus allergen, Fel d 1, Chain 1.
Purification: Produced in ascites and purified by ammonium sulphate precipitation and HPLC using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA for Fel d 1 allergen at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Universal Allergen Standard
Vial: White Cap; 400µl
Composition: A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4
Concentration/Calibration:  
Universal Allergen Standard
Protein Measurement
Concentration (ng/ml)
Der p 1
Amino-acid analysis
2500
Der f 1
Amino-acid analysis
2500
Der p 2
Amino-acid analysis
1000
Fel d 1
Amino-acid analysis
1000
Can f 1
Amino-acid analysis
2500
Rat n 1
Amino-acid analysis
1000
Mus m 1
Amino-acid analysis
250
Bla g 2
Amino-acid analysis
2500

References:

1.  Chapman MD, Aalberse RC, Brown MJ, Platts-Mills TA. Monoclonal antibodies to the major feline allergen Fel d 1. 11. Single step affinity purification of Fel d 1, N terminal sequence analysis, and development of a sensitive two-site immunoassay to assess Fel d 1 exposure. J Immunol 1988; 140(3):812-818.

2.  de Blay F, Chapman MD, Platts-Mills TA. Airborne cat allergen (Fel d 1). Environmental control with the cat in situ. Am Rev Respir Dis 1991; 143:1334-39.

3.  Ingram JM, Sporik R, Rose G, Honsinger R, Chapman MD, Platts-Mills TA. Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens: relation to sensitization and asthma among children living in Los Alamos, New Mexico. J Allergy Clin Immunol 1995; 96(4):449-456.

4.  Patchett K, Lewis S, Crane J, Fitzharris P. Cat Allergen (Fel d 1) levels on school children’s clothing and in primary school classrooms in Wellington, New Zealand. J Allergy Clin Immunol 1997; 100(6 Pt ):755-759

5.  Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24.

6.  Platts-Mills TA, Vaughan J, Squillace S, Woodfolk J, Sporik R. Sensitisation, asthma, and a modified Th2 response in children exposed to cat allergen: a population-based cross-sectional study. Lancet 2001; 357(9258):752-756.

7.  Arbes SJ, Jr.,Cohn RD, Yin M, Muilenberg ML, Friedman W, Zeldin DC. Dog allergen (Can f 1) and cat allergen (Fel d 1) in US homes: results from the National Survey of Lead and Allergens in Housing. J Allergy Clin Immunol 2004; 114(1):111- 117.

8.  Heinrich J, Bedada GB, Zock JP, Chinn S, Norback D, Oliveri M et al. Cat allergen level: its determinants and relationships to specific IgE to cat across European centers. J Allergy Clin Immunol 2006; 118(3):674-681

9.  Jarvis D, Zock JP, Heinrich J, Svanes C, Verlato G, Olivieri M et al. Cat and dust mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. J Allergy Clin Immunol. 2007 Mar; 119(3):697-704.

(For complete reference list see EL-FD1 Certificate of Analysis)

 

File Name Size Added Link
Focus on... Fel d 1 88.61KB 22/05/2014 Download
Focus on... Fel d 1 - The most potent allergen in cat dander
EL-FD1_CoA 390.71KB 21/05/2014 Download
Certificate of Analysis for Fel d 1 ELISA kit
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