Can f 1 ELISA kit (EL-CF1)

Can f 1 ELISA kit (EL-CF1)
Product Code: EL-CF1
Availability: In Stock
Price: $795.00
Qty:  

ELISA kit for measuring Canis familiaris allergen, Can f 1.

Antibodies: 10D4/pAb
Sensitivity: 0.5ng/ml
Limit of Detection: 0.5ng/ml
Assay Performance: Intra-assay CV% of 6.0; Inter-assay CV% of 9.5
Contents:

Monoclonal antibody 10D4, Universal Allergen Standard, Polyclonal Rabbit anti-Can f 1; sufficient reagents for 10 microtiter plates.

Storage: The ELISA kit should be stored at 4°C.
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Peroxidase conjugated Goat anti. Rabbit IgG
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-CF1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Monoclonal Antibody: 10D4 (clone 10D4 C4 A10)
Vial: Red Cap; 100µl
Immunogen: Can f 1
Isotype: Mouse IgG2ak
Specificity: Binds to an epitope on dog Canis familiaris allergen, Can f 1.
Purification: Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Antibody: Rabbit polyclonal antiserum
Immunogen: Dog fur extract
Vial: Brown Cap; 100µl
Specificity: The antiserum contains IgG antibodies to dog Canis familiaris allergen, Can f 1, as well as IgG antibodies to other dog allergens.
Activity: Titrated for use in ELISA at 1/1000 dilution. Prepared in 1%BSA/ 50%glycerol/ PBS, pH 7.4., 0.22μm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Universal Allergen Standard
Vial: White Cap; 400µl
Composition: A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration:  
Universal Allergen Standard
Protein Measurement
Concentration (ng/ml)
Der p 1
Amino-acid analysis
2500
Der f 1
Amino-acid analysis
2500
Der p 2
Amino-acid analysis
1000
Fel d 1
Amino-acid analysis
1000
Can f 1
Amino-acid analysis
2500
Rat n 1
Amino-acid analysis
1000
Mus m 1
Amino-acid analysis
250
Bla g 2
Amino-acid analysis
2500
References:  
1.  Larsen JN, Ford A, Gjesing B, et al. The collaborative study of the international standard of dog, Canis domesticus, hair/dander extract. J Allergy Clin Immunol 1988; 82:318-330.
2.  DeGroot H, Goei K, Van Sicter P, et al. Affinity purification of a major and minor allergen from dog extract: Serologic activity of affinity-purified Can f 1 and Can f 1-depleted extract. J Allergy Clin Immunol 1991; 87:1056-61.
3.  Schou C, Hansen G, Linter T, et al. Assay for the major dog allergen, Can f 1 investigation of house dust samples and commercial dog extracts. J Allergy Clin Immunol 1992; 88:847-53.
4.  Ingram JM, Sporik R, Rose G, Honsiger R, Chapman MD, Platts-Mills TAE. Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens: Relationship to sensitization and asthma among children living in Los Alamos, New Mexico. J Allergy Clin Immunol 1995; 96:449-456.
5.  Sporik RB, Ingram JM, Price W, Sussman JH, Honsiger RW, Platts-Mills TAE. Association of asthma with serum IgE and skin test reactivity to allergens among children living at high altitude: Tickling the dragon’s breath. Amer J Resp Crit Care Med 1995;151:1388-1392.
(For complete reference list see EL-CF1 Certificate of Analysis)

 

File Name Size Added Link
EL-CF1_CoA 155.59KB 21/05/2014 Download
Certificate of Analysis for Can f 1 ELISA kit
    54.156.67.122
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