Bos d 2 ELISA kit (EL-BD2)

Bos d 2 ELISA kit (EL-BD2)
Product Code: EL-BD2
Availability: In Stock
Price: $795.00
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ELISA kit for measuring bovine Bos domesticus dander allergen, Bos d 2.

Antibodies: 3D4/mAb1
Sensitivity: 0.1 ng/ml
Limit of Detection:

0.4 ng/ml

Contents:

Monoclonal antibody 3D4, Bos d 2 Standard, Biotinylated antibody mAb1; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:

The Bovine Allergen Standard is recommended for immunoassay calibration purposes only. Not recommended for in-vitro antibody measurements, T cell studies, immunization purposes, or other uses.

Product Resources: EL-BD2 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Monoclonal Antibody: 3D4
Vial: Red Cap; 100µl
Immunogen: Bovine dander allergen
Isotype: IgG1
Specificity:

Binds to an epitope on bovine Bos domesticus dander allergen, Bos d 2.

Purification:

Produced in tissue culture and purified by affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

Concentration:

1.33 mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.


  Certificate of Analysis
Monoclonal Antibody: mAb1
Vial: Brown Cap; 100µl
Immunogen: Bovine dander allergen
Isotype: IgG2b
Specificity:

Binds to an epitope on bovine Bos domesticus dander allergen, Bos d 2.

Purification:

Produced in tissue culture and purified by affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

Biotinylation:

Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.


  Certificate of Analysis
Allergen Standard: Bos d 2 Standard
Vial: White Cap; 400µl
Composition:

Recombinant Bos d 2 allergen was prepared in 0.5% HSA/50% glycerol/PBS, pH 7.4.

Concentration/Calibration:  500 ng/ml. The concentration of the purified recombinant Bos d 2 was determined by amino acid analysis. There are no national or international Reference standards for Bos d 2.

References:

1.  Ylönen J, Virtanen T, Rytkönen M, Mäntyjärvi R. Affinity purification of the major bovine allergen by a novel monoclonal antibody. J Allergy Clin Immunol. 1994 May;93(5):851-8.


2.  Ylönen J, Virtanen T, Rytkönen M, Mäntyjärvi R. Quantification of a major bovine allergen by a two-site immunometric assay based on monoclonal antibodies. Allergy. 1994 Oct;49(9):707-12.


3.  Rouvinen J, Rautiainen J, Virtanen T, Zeiler T, Kauppinen J, Taivainen A, Mäntyjärvi R. Probing the molecular basis of Allergy: Three-dimensional structure of the bovine lipocalin allergen Bos d 2. The Journal of Biological Chemistry 1999;274(4):2337-43.


4.  Saarelainen SA, Kinnunen TT, Buhot C, Närvänen AT, Kauppinen AK, Rytkönen-Nissinen MA, Maillere B, Virtanen TI. Immunotherapeutic potential of the immunodominant T-cell epitope of lipocalin allergen Bos d 2 and its analogues. Immunology. 2008 Mar;123(3):358-66.


5.  Filep S, Tsay A, Vailes LD, Gadermaier G, Ferreira F, Matsui EC et al. A multi-allergen standard for immunoassays: CREATE principles applied to eight purified allergens. Allergy. 18 Nov 2011; DOI: 10.1111/j. 1398-9995.2011.02750x.


6.  Kauppinen A, Peräsaari J, Taivainen A, Kinnunen T, Saarelainen S, Rytkönen-Nissinen M, Jeal H, Jones M, Virtanen T. Association of HLA class II alleles with sensitization to cow dander Bos d 2, an important occupational allergen. Immunobiology. 2012 Jan; 217(1):8-12.

(For complete reference list see EL-BD2 Certificate of Analysis)

 

File Name Size Added Link
EL-BD2 CoA 379.91KB 28/05/2014 Download
Certificate of Analysis for Bos d 2 ELISA kit
    54.156.67.122
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