Mus m 1 ELISA kit (EL-MM1)

Mus m 1 ELISA kit (EL-MM1)
Product Code: EL-MM1
Availability: In Stock
Price: $795.00
Qty:  

ELISA kit for measuring Mouse Urinary Protein (MUP) allergen, Mus m 1.

Antibodies: pAb/pAb
Sensitivity: 0.05ng/ml
Limit of Detection: 0.2ng/ml
Assay Performance: Intra-assay CV% of 6.1; Inter-assay CV% of 14.4
Contents:

Rabbit anti. Mus m 1, Universal Allergen Standard, Biotinylated Rabbit anti. Mus m 1; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-MM1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

   
  Certificate of Analysis
Polyclonal Antibody: Rabbit polyclonal antiserum
Vial: Red Cap; 100µl
Immunogen: Mus m 1
Isotype: Multiple
Specificity: Binds to an epitope on mouse Mus musculus urinary protein allergen, Mus m 1.
Purification: Affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
Concentration: 1.6mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

  Certificate of Analysis
Polyclonal Antibody: Rabbit polyclonal antiserum
Vial: Brown Cap; 100µl
Immunogen: Mus m 1
Isotype: Multiple
Specificity: Binds to an epitope on mouse Mus musculus urinary protein allergen, Mus m 1.
Purification: Affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Universal Allergen Standard
Vial: White Cap; 400µl
Composition: A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4
Concentration/Calibration:  
Universal Allergen Standard
Protein Measurement
Concentration (ng/ml)
Der p 1
Amino-acid analysis
2500
Der f 1
Amino-acid analysis
2500
Der p 2
Amino-acid analysis
1000
Fel d 1
Amino-acid analysis
1000
Can f 1
Amino-acid analysis
2500
Rat n 1
Amino-acid analysis
1000
Mus m 1
Amino-acid analysis
250
Bla g 2
Amino-acid analysis
2500

References: 

1. Phipatanakul W, Eggleston PA, Wright EC, Wood RA.  Mouse allergen. I. The prevalence of mouse allergen in inner-city homes. The National Cooperative Inner-City Asthma Study.  J Allergy Clin Immunol. 2000;106:1070-4. 

2. Phipatanakul W, Eggleston PA, Wright EC, Wood RA.  Mouse allergen. II. The relationship of mouse allergen exposure to mouse sensitization and asthma morbidity in inner-city children with asthma.  J Allergy Clin Immunol. 2000;106:1075-80.

3. Permaul P, Hoffman E, Fu C, Sheehan W, Baxi S, Gaffin J, Lane J, Bailey A, King E, Chapman M, Gold D, Phipatanakul W.  Allergens in urban schools and homes of children with asthma.  Pediatr Allergy Immunol. 2012;23:543-9.

4.  Ahluwalia SK, Peng RD, Breysse PN, Diette GB, Curtin-Brosnan J, Aloe C, Matsui EC.  Mouse allergen is the major allergen of public health relevance in Baltimore City.  J Allergy Clin Immunol. 2013;132:830-5.

5.  Ownby DR.  Will the real inner-city allergen please stand up?  J Allergy Clin Immunol. 2013;132:836-7.

(For complete reference list see EL-MM1 Certificate of Analysis)

 

File Name Size Added Link
EL-MM1_CoA 387.37KB 22/05/2014 Download
Certificate of Analysis for Mus m 1 ELISA kit
    54.166.19.237
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